M103a
Modified Oxford Listeria Selective Agar
|
M103a
Modified Oxford Listeria Selective Agar
| Columbia Blood Agar Base (brand dependent) | 39.0-44.0 g |
| Agar | 2.0 g |
| Esculin | 1.0 g |
| Ferric ammonium citrate | 0.5 g |
| Lithium chloride (Sigma L0505 quality or equivalent) | 15.0 g |
| Buffered colistin methane sulfonate (1 % w/v) solution | 1.0 ml |
| Distilled water | 1.0 L |
Hydrate the basal medium powder by stirring gradually into distilled water. Adjust pH to 7.2±0.1 if need be. Autoclave at 121° C for 10 min. Mix again. Cool rapidly to 46 ° C in a waterbath. Add 2 ml of 1 % w/v filter sterilized buffered moxalactam solution and mix well. Pour 12 ml per Petri dish.
Buffered 1% w/v colistin solution:
| Colistin methane sulfonate (Sigma C1511 quality or equivalent) | 1.0 g |
| 0.1 M Potassium phosphate buffer, pH 6.0±0.1 | 100 ml |
Dispense buffered solution as 5 mL aliquots. Store aliquots at -20° C.
Buffered 1% w/v moxalactam solution:
| Sodium (or ammonium) moxalactam (Sigma M1900 quality or equivalent) | 1.0 g |
| 0.1 M Potassium phosphate buffer, pH 6.0±0.1 | 100 ml |
Dissolve, filter-sterilize, and dispense as 2-ml aliquots. Store at -20° C.
