R84
Tris-Buffered Saline (TABS), 1% or 3% Gelatin, or Tween 20
|
R84
Tris-Buffered Saline (TABS), 1% or 3% Gelatin, or Tween 20
| Tris | 2.42 g |
| NaCl | 29.24 g |
| Distilled water | 1 liter |
Dissolve ingredients in distilled water by heating and stirring. Adjust pH to 7.5 with HC1. Autoclave 15 min at 121°C.
For 1% and 3% Gelatin-TABS, add 10 g and 30 g gelatin, respectively, to ingredients before autoclaving. Adjust final pH to 7.5 with HCl.
For Tween-TABS, add 0.5 ml Tween 20 to ingredient and adjust pH to 7.5 before autoclaving.
Hypertext updated by rim 2000-APR-12
