U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R45
Methylene Blue Stain (Loeffler's)

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Solution A


Methylene blue (90% dye content) 0.3 g
Ethanol (95%) 30 ml


Solution B


Diluted potassium hydroxide (0.01%)

100 ml


 

Mix solutions A and B.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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