U.S. Food & Drug Administration
Center for
Food
Safety &
Applied
Nutrition
Bacteriological
Analytical
Manual Online
January 2001
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R35
Hybridization Mixture
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(6X SSC, 5X Denhardt's, 1 mM EDTA, pH 8.0)
| 20X SSC |
15.0 ml |
| 50X Denhardt's solution |
5.0 ml |
| 0.5 M EDTA |
0.1 ml |
| Distilled water |
28.9 ml |
For hybridization with 10X Denhardt's, add 10 ml 50X Denhardt's, and decrease water volume to 23.9 ml.
Hypertext Source: Bacteriological Analytical
Manual, 8th Edition, Revision A, 1998.
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