Colocalization

Colocalization_Finder

Authors: Christophe Laummonerie,
Jerome Mutterer, Institut de Biologie Moleculaire des Plantes, Strasbourg, France.

History: 2004/02/02: First version
2005/01/28: Version 1.1 : statistical methods added.
2006/08/29: Version 1.2
- ratio bars non destructively displayed
- works with any closed ROI
- Fire LUT better diplayed
- You now have to click in the ROI to update overaly image(s) and statistics

Source: Colocalization_Finder.java in the Jar file.

Installation: Download Colocalization_Finder.jar to the plugins folder and restart ImageJ.

Description: This plugin displays a correlation diagram for two images (8bits, same size). Drawing a rectangular selection on this diagram allows you to highlight corresponding pixels on a RGB overlap of the original images, and if selected, on a 3rd image. Analysis can be restricted to pixels having values with a minimum ratio. Selection settings are logged to a results window. Parts of this code were taken from plugins by W. Rasband, P. Bourdoncle, and G. Chinga.
Example results:

The results table with computer Pearson's correlation, Overlap coefficient, contribution of both channels to OC, Slope and intercept of the linear regression,% pixels selected, min and max in both channels.

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Authors:	Christophe Laummonerie, Jerome Mutterer, Institut de Biologie Moleculaire des Plantes, Strasbourg, France.
History:	2004/02/02: First version 2005/01/28: Version 1.1 : statistical methods added. 2006/08/29: Version 1.2 - ratio bars non destructively displayed - works with any closed ROI - Fire LUT better diplayed - You now have to click in the ROI to update overaly image(s) and statistics
Source:	Colocalization_Finder.java in the Jar file.
Installation:	Download Colocalization_Finder.jar to the plugins folder and restart ImageJ.
Description:	This plugin displays a correlation diagram for two images (8bits, same size). Drawing a rectangular selection on this diagram allows you to highlight corresponding pixels on a RGB overlap of the original images, and if selected, on a 3rd image. Analysis can be restricted to pixels having values with a minimum ratio. Selection settings are logged to a results window. Parts of this code were taken from plugins by W. Rasband, P. Bourdoncle, and G. Chinga. Example results: The results table with computer Pearson's correlation, Overlap coefficient, contribution of both channels to OC, Slope and intercept of the linear regression,% pixels selected, min and max in both channels.