Nitrobenzene (CASRN 98-95-3)
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0079
Nitrobenzene;
CASRN 98-95-3
Health assessment information on a chemical substance is included in IRIS
only after a comprehensive review of chronic toxicity data by U.S. EPA
health scientists from several Program Offices and the Office of Research
and Development. The summaries presented in Sections I and II represent
a consensus reached in the review process. Background information and
explanations of the methods used to derive the values given in IRIS are
provided in the Background Documents.
STATUS OF DATA FOR Nitrobenzene
File First On-Line 01/31/1987
Category (section) |
Status |
Last Revised |
---|---|---|
Oral RfD Assessment (I.A.) | on-line | 01/01/1991 |
Inhalation RfC Assessment (I.B.) | no data | |
Carcinogenicity Assessment (II.) | on-line | 02/01/1995 |
_I. Chronic Health Hazard Assessments for Noncarcinogenic Effects
_I.A. Reference Dose for Chronic Oral Exposure (RfD)
Substance Name — Nitrobenzene
CASRN — 98-95-3
Last Revised — 01/01/1991
The oral Reference Dose (RfD) is based on the assumption that thresholds
exist for certain toxic effects such as cellular necrosis. It is expressed
in units of mg/kg-day. In general, the RfD is an estimate (with uncertainty
spanning perhaps an order of magnitude) of a daily exposure to the human
population (including sensitive subgroups) that is likely to be without
an appreciable risk of deleterious effects during a lifetime. Please refer
to the Background Document for an elaboration of these concepts. RfDs
can also be derived for the noncarcinogenic health effects of substances
that are also carcinogens. Therefore, it is essential to refer to other
sources of information concerning the carcinogenicity of this substance.
If the U.S. EPA has evaluated this substance for potential human carcinogenicity,
a summary of that evaluation will be contained in Section II of this file.
NOTE: The Oral RfD for nitrobenzene may change in the near future pending the outcome of a further review now being conducted by the Oral RfD Work Group.
__I.A.1. Oral RfD Summary
Critical Effect |
Experimental Doses* |
UF |
MF |
RfD |
---|---|---|---|---|
Hematologic, adrenal, Rat/Mouse Subchronic CIIT, 1984 |
NOAEL: none LOAEL: 25 mg/cu.m |
10,000 |
1 |
5E-4 mg/kg/day |
*Conversion Factors: 6 hour/24 hour, 5 days/7 days, 0.039 cu.m/day/0.03 kg (mice breathing rate/body weight) and 0.8 absorption factor; thus, 25 mg/cu.m x 6 hour/24 hour x 5 days/7 days x 0.039 cu.m/day / 0.03 kg x 0.8 = 4.6 mg/kg/day
__I.A.2. Principal and Supporting Studies (Oral RfD)
CIIT (Chemical Industry Institute of Toxicology). 1984. Ninety-day inhalation toxicity study of nitrobenzene in F344 rats and B6C3F1 mice. Research Triangle Park, NC. FYI-OTS-0874-0333.
The CIIT 90-day inhalation study provides the most appropriate data currently available to derive an RfD. Ten animals/sex/species/dose group were administered nitrobenzene at 1 of 3 doses. Other than increased incidence of hemolytic anemia in rats at 25 mg/cu.m and vacuolization of adrenal cortical cells in female mice at 25 mg/cu.m and higher, adverse effects of nitrobenzene exposure in mice and rats were comparable to unexposed controls at this dose. Mice and rats exposed to nitrobenzene at 81 mg/cu.m showed increased incidence and severity of liver and kidney lesions.
Data regarding the effects of nitrobenzene in humans are limited to symptoms and observations in workers, including headaches, vertigo, and methemoglobinemia (ACGIH, 1980). The potential "safe" level derived from the TLV appears adequate to protect workers from such adverse effects; however, the effects of occupational exposure to nitrobenzene on the liver and/or kidneys have not been adequately evaluated. The CIIT (1984) study indicates that the liver and kidney may be target organs of chronic/subchronic nitrobenzene exposure, and an acceptable level based on the TLV may not be protective for the toxic effects of nitrobenzene on the liver and/or kidney. Therefore, until more definitive chronic data are available, the RfD of 0.0005 mg/kg/day is recommended to protect against adverse health effects of nitrobenzene.
__I.A.3. Uncertainty and Modifying Factors (Oral RfD)
UF — The uncertainty factor of 10,000 represents two 10-fold factors for both intra- and interspecies variability to the toxicity of this chemical in lieu of specific data, a 10-fold factor for estimating a chronic effect level, from its subchronic equivalent, and a 10-fold factor for estimating an RfD from a LOAEL rather than a NOAEL.
MF — None
__I.A.4. Additional Studies/Comments (Oral RfD)
None.
__I.A.5. Confidence in the Oral RfD
Study — Medium
Database — Low
RfD — Low
Medium to low confidence in the study is recommended because it is not an oral study, a limited number of animals/sex/dose were tested, and a NOAEL for the critical toxic effect (i.e., adrenal toxicity) was not determined, although two species were used and many parameters were measured. Low confidence in the database is recommended because chronic reproductive and teratology data are missing. Low confidence in the RfD follows.
__I.A.6. EPA Documentation and Review of the Oral RfD
Source Document — U.S. EPA, 1985
Other EPA Documentation — None
Agency Work Group Review — 06/24/1985, 07/08/1985, 11/06/1985
Verification Date — 07/08/1985
__I.A.7. EPA Contacts (Oral RfD)
Please contact the IRIS Hotline for all questions concerning this assessment or IRIS, in general, at (301)345-2870 (phone), (301)345-2876 (FAX) or hotline.iris@epa.gov (internet address).
_I.B. Reference Concentration for Chronic Inhalation Exposure (RfC)
Substance Name — Nitrobenzene
CASRN — 98-95-3
Not available at this time.
_II. Carcinogenicity Assessment for Lifetime Exposure
Substance Name — Nitrobenzene
CASRN — 98-95-3
Last Revised — 02/01/1995
Section II provides information on three aspects of the carcinogenic assessment for the substance in question; the weight-of-evidence judgment of the likelihood that the substance is a human carcinogen, and quantitative estimates of risk from oral exposure and from inhalation exposure. The quantitative risk estimates are presented in three ways. The slope factor is the result of application of a low-dose extrapolation procedure and is presented as the risk per (mg/kg)/day. The unit risk is the quantitative estimate in terms of either risk per ug/L drinking water or risk per ug/cu.m air breathed. The third form in which risk is presented is a drinking water or air concentration providing cancer risks of 1 in 10,000, 1 in 100,000 or 1 in 1,000,000. The rationale and methods used to develop the carcinogenicity information in IRIS are described in The Risk Assessment Guidelines of 1986 (EPA/600/8-87/045) and in the IRIS Background Document. IRIS summaries developed since the publication of EPA's more recent Proposed Guidelines for Carcinogen Risk Assessment also utilize those Guidelines where indicated (Federal Register 61(79):17960-18011, April 23, 1996). Users are referred to Section I of this IRIS file for information on long-term toxic effects other than carcinogenicity.
NOTE: The carcinogenicity assessment for nitrobenzene may change in the near future pending the outcome of a further review now being conducted by the Carcinogen Risk Assessment Verification Endeavor Work Group.
_II.A. Evidence for Human Carcinogenicity
__II.A.1. Weight-of-Evidence Characterization
Classification — D; not classifiable as to human carcinogenicity
Basis — Based on no data concerning carcinogenicity in humans or animals.
__II.A.2. Human Carcinogenicity Data
None.
__II.A.3. Animal Carcinogenicity Data
None.
__II.A.4. Supporting Data for Carcinogenicity
In Salmonella/microsomal mutagenicity assays with strains TA92, TA94, TA97, TA98, TA100, TA1535, TA1537 and TA1538, nitrobenzene was not positive (Garner and Nutman, 1977; Chiu et al., 1978; Shimizu et al., 1983; Ho et al., 1981; Haworth et al., 1983; Anderson and Styles, 1978; Miyata et al., 1981). These assays were conducted with and without liver homogenates by plate incorporation, spot test and in one study (Hughes et al., 1984), by the vapor exposure method.
Nitrobenzene was mutagenic in the presence of liver homogenate and norharman in S. typhimurium strain TA98 but not in strain TA100 (Suzuki et al., 1983). Nitrobenzene did not cause an increase in unscheduled DNA synthesis in hepatocytes isolated from gavage-treated rats (Mirsalis et al., 1982). Fel'dt (1985) reported that oral administration of nitrobenzene did not produce micronucleus or chromosome aberrations in bone marrow cells or dominant lethal mutations in mice.
_II.B. Quantitative Estimate of Carcinogenic Risk from Oral Exposure
None.
_II.C. Quantitative Estimate of Carcinogenic Risk from Inhalation Exposure
None.
_II.D. EPA Documentation, Review, and Contacts (Carcinogenicity Assessment)
__II.D.1. EPA Documentation
U.S. EPA. 1985. Health and Environmental Effects Profile for Nitrobenzene. Prepared by the Office of Health and Environmental Assessment, Environmental Criteria and Assessment Office, Cincinnati, OH, for the Office of Solid Waste and Emergency Response, Washington, DC.
U.S. EPA. 1987. Health Effects Assessment for Nitrobenzene. Prepared by the Office of Health and Environmental Assessment, Environmental and Criteria and Assessment Office, Cincinnati, OH, for the Office of Solid Waste and Emergency Response, Washington, DC.
The 1985 Health and Environmental Effects Profile for Nitrobenzene and the 1987 Health Effects Assessment for Nitrobenzene have received OHEA review.
__II.D.2. EPA Review (Carcinogenicity Assessment)
Agency Work Group Review — 11/08/1989, 12/07/1994
Verification Date — 11/08/1989
__II.D.3. EPA Contacts (Carcinogenicity Assessment)
Please contact the IRIS Hotline for all questions concerning this assessment or IRIS, in general, at (301)345-2870 (phone), (301)345-2876 (FAX) or hotline.iris@epa.gov (internet address).
_III.
[reserved]
_IV. [reserved]
_V. [reserved]
_VI. Bibliography
Substance Name — Nitrobenzene
CASRN — 98-95-3
Last Revised — 12/01/1990
_VI.A. Oral RfD References
ACGIH (American Conference of Governmental Industrial Hygienists). 1980. Documentation of the Threshold Limit Values. 4th ed. Cincinnati, OH. p. 303.
CIIT (Chemical Industry Institute of Toxicology). 1984. Ninety-day inhalation toxicity study of nitrobenzene in F344 rats, CD rats, and B6C3F1 mice. Research Triangle Park, NC. FYI-OTS-0784-0333.
U.S. EPA. 1985. Health and Environmental Effects Profile for Nitrobenzene. Prepared by the Office of Health and Environmental Assessment, Environmental Criteria and Assessment Office, Cincinnati, OH for the Office of Solid Waste and Emergency Response, Washington, DC.
_VI.B. Inhalation RfC References
None
_VI.C. Carcinogenicity Assessment References
Anderson, D. and J.A. Styles. 1978. An evaluation of 6 short-term tests for detecting organic chemical carcinogens. Appendix II. The bacterial mutation test. Br. J. Cancer. 37: 924-930.
Chiu, C.W., L.H. Lee, C.Y. Wang and G.T. Bryan. 1978. Mutagenicity of some commercially available nitro compounds or Salmonella typhimurium. Mutat. Res. 58: 11-22.
Fel'dt, E.G. 1985. Evaluation of the mutagenic Hazards of benzene and some of its derivatives. Gig. Sanit. 7: 21-23.
Garner, R. and C.A. Nutman. 1977. Testing of some azo dyes and their reduction products for mutagenicity using Salmonella typhimurium TA1538. Mutat. Res. 44: 9-19.
Haworth, S., T. Lawlor, K. Mortelmans, W. Speck and E. Zeiger. 1983. Salmonella mutagenicity test results for 250 chemicals. Environ. Mutagen.(Suppl.) 1: 3-142.
Ho, C.H., B.R. Clark, M.R. Guerin, B.D. Barkenbus, T.K. Rao and J.L. Epler. 1981. Analytical and biological analyses of test materials from the synthetic fuel technologies. IV. Studies of chemical structure-mutagenic activity relationships of aromatic nitrogen compounds relevant to synfuels. Mutat. Res. 85(5): 335-345.
Hughes, T.J., C. Sparacino and S. Frazier. 1984. Validation of chemical and biological techniques for evaluation of vapors in ambient air/mutagenicity testing of twelve (12) vapor-phase compounds. EPA 600/1-84-005. NTIS PB 84-164219.
Mirsalis, J.C., C.K. Tyson and B.E. Butterworth. 1982. Detection of genotoxic carcinogens in the in vivo-in vitro hepatocyte DNA repair assay. Environ. Mutagen. 4: 553-562.
Miyata, R., T. Nomi, K. Yoshikawa and M. Ishidate. 1981. Metabolic activation of p- nitrotoluene and trichloroethylene by rat liver S9 or mouse liver S9 fractions in Salmonella typhimurium strains. Eisei Shikensho Hokoku. 99: 60-65.
Shimizu, M., Y. Yasui and N. Matsumoto. 1983. Structural specificity of aromatic compounds with special reference to mutagenic activity in Salmonella typhimurium: A series of chloro- or fluoro-nitrobenzene derivatives. Mutat. Res. 116: 217-238.
Suzuki, J., T. Koyami and S. Suzuki. 1983. Mutagenicities of mono-nitrobenzene derivatives in the presence of norharman. Mutat. Res. 120: 105-110.
U.S. EPA. 1985. Health and Environmental Effects Profile for Nitrobenzene. Prepared by the Office of Health and Environmental Assessment, Environmental Criteria and Assessment Office, Cincinnati, OH, for the Office of Solid Waste and Emergency Response, Washington, DC.
U.S. EPA. 1987. Health Effects Assessment for Nitrobenzene. Prepared by the Office of Health and Environmental Assessment, Environmental and Criteria and Assessment Office, Cincinnati, OH, for the Office of Solid Waste and Emergency Response, Washington, DC.
_VII. Revision History
Substance Name — Nitrobenzene
CASRN — 98-95-3
Date |
Section |
Description |
---|---|---|
03/01/1988 | I.A.2. | Text deleted |
03/01/1988 | I.A.4. | Text deleted |
03/01/1988 | I.A.5. | Confidence levels revised |
03/01/1988 | I.A.7. | Documentation corrected |
06/30/1988 | I.A.3. | UF corrected in text |
07/01/1989 | I.B. | Inhalation RfD now under review |
08/01/1989 | I.A. | Oral RfD summary noted as pending change |
12/01/1989 | II. | Carcinogen assessment now under review |
06/01/1990 | IV.F.1. | EPA contact changed |
06/01/1990 | VI. | Bibliography on-line |
12/01/1990 | II. | Carcinogen assessment on-line |
12/01/1990 | VI.C. | Carcinogen assessment references added |
01/01/1991 | I.A. | Text edited |
01/01/1992 | I.A.7. | Primary contact changed |
01/01/1992 | IV. | Regulatory actions updated |
01/01/1995 | II.D.2. | Work group review date added |
02/01/1995 | II. | Carcinogenicity assessment noted as pending change |
08/01/1995 | I.A., I.B., II., II.D.2. | EPA's RfD/RfC and CRAVE workgroups were discontinued in May, 1995. Chemical substance reviews that were not completed by September 1995 were taken out of IRIS review. The IRIS Pilot Program replaced the workgroup functions beginning in September, 1995. |
04/01/1997 | III., IV., V. | Drinking Water Health Advisories, EPA Regulatory Actions, and Supplementary Data were removed from IRIS on or before April 1997. IRIS users were directed to the appropriate EPA Program Offices for this information. |
01/02/1998 | I., II. | This chemical is being reassessed under the IRIS Program. |
_VIII. Synonyms
Substance Name — Nitrobenzene
CASRN — 98-95-3
Last Revised — 01/31/1987
- 98-95-3
- Benzene, Nitro-
- Essence of Mirbane
- Essence of Myrbane
- Mirbane Oil
- NCI-C60082
- Nitrobenzene
- Nitrobenzol
- Oil of Mirbane
- Oil of Myrbane