M24
Brain Heart Infusion (BHI) Broth and Agar(Equivalent media are available from Difco and BBL)
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M24
Brain Heart Infusion (BHI) Broth and Agar(Equivalent media are available from Difco and BBL)
| Calf brain, infusion from | 200 g |
| Beef heart, infusion from | 250 g |
| Proteose peptone (Difco) or polypeptone (Bioquest) |
10 g |
| NaCl | 5 g |
| Na2HPO4* | 2.5 g |
| Dextrose | 2.0 g |
| Distilled water | 1 liter |
Dissolve ingredients of Medium 1 in distilled water with gentle heat.
*Difco does not specify waters of hydration.
| Brain heart-infusion | 6.0 g |
| Peptic digest of animal tissue | 6.0 g |
| NaCl | 5.0 g |
| Dextrose | 3.0 g |
| Pancreatic digest of gelatin | 14.5 g |
| Na2HPO4 + | 2.5 g |
| Distilled water | 1 liter |
Suspend ingredients of Medium 2 in distilled water and boil for 1 min to completely dissolve.
+BBL does not specify waters of hydration.
For both Medium 1 and Medium 2, dispense broth into bottles or tubes for storage. Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2. Commercially available BHI is acceptable.
To prepare brain heart infusion agar, add 15 g agar to 1 liter BHI broth. Heat to dissolve agar before dispensing into bottles or flasks. Autoclave 15 min at 121°C.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21
