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M19
Bismuth Sulfite Agar
(Wilson and Blair)
Polypeptone (or peptone) | 10 g |
Beef extract | 5 g |
Dextrose | 5 g |
Na2HPO4 (anhydrous) | 4 g |
FeSO4 (anhydrous) | 0.3 g |
Bismuth sulfite (indicator) | 8 g |
Brilliant green | 0.025 g |
Agar | 20 g |
Distilled water | 1 liter |
Mix thoroughly and heat with agitation. Boil about 1 min to obtain uniform suspension. (Precipitate will not dissolve.) Cool to 45-50°C. Suspend precipitate by gentle agitation, and pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let plates dry about 2 h with lids partially removed; then close plates. Final pH, 7.7 ± 0.2
DO NOT AUTOCLAVE. Prepare plates on day before streaking and store in dark. Selectivity decreases in 48 h.
CAUTION | DO NOT AUTOCLAVE |
PREPARATION | Day before use. |
STORAGE | In Dark |
Loss of Selectivity after 48 h |
Hypertext updated by rim/cjm 2005-OCT-21