M149
Triple Sugar Iron Agar (TSI)
|
M149
Triple Sugar Iron Agar (TSI)
Medium 1 |
Medium 2 |
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| Polypeptone | 20 g | Beef extract | 3 g |
| NaCl | 5 g | Yeast extract | 3 g |
| Lactose | 10 g | Peptone | 15 g |
| Sucrose | 10 g | Proteose peptone | 5 g |
| Glucose | 1 g | Glucose | 1 g |
| Fe(NH4)2(SO4)2•6H2O | 0.2 g | Lactose | 10 g |
| Na2S2O3 | 0.2 g | Sucrose | 10 g |
| Phenol red | 0.025 g | FeSO4 | 0.2 g |
| Agar | 13 g | NaCl | 5 g |
| Distilled water | 1 liter | Na2S2O3 | 0.3 g |
| Phenol red | 0.024 g | ||
| Agar | 12 g | ||
| Distilled water | 1 liter | ||
These two media are interchangeable for general use.
Suspend ingredients of Medium 1 in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min to dissolve ingredients. Fill 16 x 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave Medium 1 for 15 min at 118°C.
Prepare Medium 2 in the same manner as Medium 1, except autoclave 15 min at 121°C. Before the media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt. Final pH, 7.3 ± 0.2 for Medium 1 and 7.4 ± 0.2 for Medium 2.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21
