U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M145
Tetrathionate (TT) Broth

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Tetrathionate broth base


Polypeptone 5 g
Bile salts 1 g
Calcium carbonate 10 g
Sodium thiosulfate.5H2O 30 g
Distilled water 1 liter

 

Suspend ingredients in 1 liter distilled water, mix, and heat to boiling. DO NOT AUTOCLAVE. (Precipitate will not dissolve completely.) Cool to less than 45°C. Store at 5-8°C. Final pH, 8.4 ± 0.2.


 

Iodine-Potassium Iodide (I2-KI) solution


Potassium iodide 5 g
Iodine, resublimed 6 g
Distilled water, sterile 20 ml

Dissolve potassium iodide in 5 ml sterile distilled water. Add iodine and stir to dissolve. Dilute to 20 ml.


 

Brilliant green solution

 

Brilliant green dye, sterile 0.1 g
Distilled water, sterile 100 m1

 

On day of use, add 20 ml I2-KI solution and 10 ml brilliant green solution to 1 liter base. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 x 150 or 16 x 150 mm sterile test tubes. Do not heat medium after addition of I2-KI and dye solutions.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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