U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M138
Simmons Citrate Agar

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Sodium citrate* 2 g
NaCl 5 g
K2HPO4 1 g
NH4H2PO4 1 g
MgSO4 0.2 g
Bromthymol blue 0.08 g
Agar 15 g
Distilled water 1 liter

 

 

Heat gently with occasional agitation. Boil 1-2 min until agar dissolves. Fill 13 x 100 or 16 x 150 mm screw-cap tubes 1/3 full. Autoclave 15 min at 121°C. Before medium solidifies, incline tubes to obtain 4-5 cm slants and 2-3 cm butts. Final pH, 6.8 ± 0.2.

 

*Difco does not specify waters of hydration.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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