U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R60
O.01 M Phosphate-Buffered Saline (pH 7.5)

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Stock solution (0.1 M)

Na2HPO4 (anhydrous) 12.0 g
NaH2PO4 · H20 2.2 g
NaCl 85.0 g
Distilled water 1 liter

 

 

Dissolve ingredients in distilled water and bring volume to 1 liter.



Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary. Commercially available in dehydrated form from Difco Laboratories and BBL.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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