|
R38
Kovacs' Reagent
p-Dimethylaminobenzaldehyde | 5 g |
Amyl alcohol (normal only) | 75 ml |
HCl (concentrated) | 25 ml |
Dissolve p-dimethylaminobenzaldehyde in normal amyl alcohol. Slowly add HCl. Store at 4°C. To test for indole, add 0.2-0.3 ml reagent to 5 ml of 24 h bacteria culture in tryptone broth. Dark red color in surface layer is positive test for indole.
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