Center for Cancer Research NCI-Frederick
  
 


Cryopreservation and Assisted Reproduction Laboratory - Request for Services

Please note that a separate form needs to be completed for each strain.

Contact Information

 
Requestor:
Principal Investigator:
Phone No: Fax No:
Email:
Institute:
Lab/Section:
Building: Room:
 

Type of Service(s) Requested
 
Cryopreservation: Embryo Sperm Ovary
 
Reproduction: Colony Expansion Special Rederivation
 
Strain Reconstitution from: Embryos Sperm Ovary
 

Laboratory Animal Model to be Sent or Reconstituted
 
Species:
Strain Nomenclature:
Short Name:
Coat Color: Age:
  Transgenic Knockout Mutant
Strain Background:
  Hybrid Inbred Mixed
Phenotypic Expression:
Is your strain genetically engineered?

Yes No

Does this construct contain a viral sequence or other potentially transposable agent?
Yes No
If yes, please describe:
Genotype:  Heterozygous Homozygous
Indicate best method to verify genotypic expression:
Method of breeding and reproductive performance:
Special Requirements/ Notes:
 

PCR Information
 
Beginning Fiscal Year 2005, the cryopreservation and Assisted Reproduction laboratory will be offering genotyping by PCR that follows the quality control procedure for sperm, embryo, and ovary cryopreservation. This is to expedite the genotyping process and to reduce turn around time for all cryopreservation requests. For this purpose, please provide the primers along with the following information:
Sequence of the Primers:
Concentration of the Primers:
Melting Temperature (Tm), °C:
Expected size of the amplified fragment:
A representative picture of the PCR product:
 

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