Clone Info
Sequencing Info
MGC Info
Other Species Collections
Useful Links
MGC Project Summary
Overview
The NIH Mammalian Gene Collection (MGC) program is a multi-institutional effort to identify and sequence cDNA clones containing a full-length open reading frame (FL-ORF) for human, mouse, and rat genes. To date, the MGC has produced over 630 cDNA libraries derived from human tissue and cell lines, as well as mouse and rat tissues. The MGC has sequenced and verified the complete FL-ORFs for a non-redundant set of 17,526 human, 17,496 mouse, and 6,457 rat genes.
MGC infrastrucure and protocols are also being applied to two other gene collection projects:
The Bovine Gene Collection (BGC) is a Genome Canada initiative that supports the production of cDNA libraries, clones and sequences to provide a complete set of full-length (open reading frame) sequences and cDNA clones of expressed genes for the cow. The MGC infrastructure provides materials distribution. Details about this project can be found at http://www.genomebc.ca/GBCResearch/researchProjectsDetails.asp?id=i1p01.
Strategy
- Clone generation:
- From the inception of the MGC, 5' expressed-sequence tags (ESTs) were generated from cDNA libraries and analyzed to identify candidate clones for human, mouse and rat that might contain complete ORFs. (See references 1, 4, and 5)
- Since 2004, PCR generation and in vitro synthesis of human and mouse cDNAs have been performed to isolate genes missing from the MGC. (See references 2 and 3)
- All clones are subjected to high accuracy full-insert sequencing and assessed for complete ORFs.
- Candidate clones for genes are clones that have been identified as potentially complete ORFs and are awaiting full-insert sequencing.
- All MGC sequences are deposited in GenBank and available without restriction.
- The cDNA clones generated by the MGC are available through the IMAGE clone distribution network (See Where to Buy) and are fully accessible to the community.