Ph.D. Chemistry, University of Michigan, Ann Arbor, Michigan
Robert Welch Postdoctoral Fellow, University of Texas Southwestern Medical Center, Dallas, Texas
Research Summary
Dr. Bienstock's research interests are in the area of computational biology and in the application of computational methods to structural biology and structure-based drug design. She applies various computational methods for protein tertiary structure prediction and to study protein dynamics and protein-protein and protein-DNA interactions. She is also interested in structure-based and ligand-based drug design methodologies such as QSAR and pharmacophore analysis and ligand docking.
Recent Projects:
DNA Repair and Replication: Modeling DNA Polymerase Gamma
Modeling Protein-protein and protein-domain interactions
Sjorgen’s syndrome protein domain interactions
Using experimental information from Mass Spectrometry as constraints in Molecular Modeling
Modeling neuronal nicotinic acetylcholine receptors and interactions with ApoE peptidic inhibitors
Recent Publications:
M. A. Graziewicz, R. J.Bienstock, W .C. Copeland, " The DNA polymerase g Y955C disease variant associated with PEO and parkinsonism mediates the incorporation and translesion synthesis opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine", Human Molecular Genetics, published on-line; doi: 10.1093/hmg/ddm227, August 27, 2007.
E.A. Gay, R. J. Bienstock, P.W. Lamb, J. L. Yakel, “Structural determinates for apolipoproteinE–derived peptide interaction with the a7 nicotinic acetylcholine receptor”, Molecular Pharmacology, published on-line; doi: 10.1124/mol.107.03552, July 3, 2007.
S. Ferraris, S. Clark, G. Davidzon, S. A. Moore, R. H. Kardon, R. J. Bienstock, M. J. Longley, M. Mancuso, M. Hirano, W. C. Copeland, and S. DiMauro, “Progressive External Ophthalmoplegia and Hearing Loss in a Patient with a Mutation in Polg”, Archives in Neurology, accepted, May 2007, in press.
L.J. Lewis-Tuffin, C. M. Jewell, R.J. Bienstock, J.B. Collins, J.A. Cidlowski, " The Human Glucocorticoid Receptor {beta}(hGR{beta}) Binds RU-486 and is Transcriptionally Active” , Mol. Cell. Biol., 27(6), 2266-2282, March 2007.
D. D. Dong, C .M. Jewell, R. J. Bienstock, J. A. Cidlowski, "Functional analysis of the LXXLL motifs of the human glucocorticoid receptor: Association with altered ligand affinity ", J Steroid Biochem Mol Biol. 101(2-3):106-17, October 2006.
D.L. Croteau, M.J. Della Vecchia, H. Wong, R. J. Bienstock, M. Melton, B. Van Houten, " The C-terminal zinc finger of UvrA does not bind DNA directly, but regulates damage-specific DNA binding", J.Biol.Chem. 281(36):26370-81, September 2006.
W. Lewis, J. J. Kohler, S. H. Hosseini, C. P. Haase, W. C. Copeland, R. J. Bienstock, T. Ludaway, J. McNaught, R. Russ, T. Stuart, and R. Santoianni ,"Pyrimidine Nucleoside Analogs Deplete mtDNA AND Polypeptides and Corroborate the DNA POL g Hypothesis", AIDS, 20(5), 675-684, March 21, 2006.
M.J. Longely, M.A. Graziewicz, R.J. Bienstock, W.C. Copeland, "Consequences of mutations in the gene for human DNA polymerase g", Gene, 354, 125-131, July 18, 2005.
L. Qin, M. L. Block, Y. Liu, R. J. Bienstock, Z. Pei, W. Zhang, X. Wu, B. Wilson, T. Burka, J.S. Hong, "Microglial NADPH Oxidase is a Novel Target for Femtomolar Neuroprotection Against Oxidative Stress", Faseb J., 19(6), April 2005.
M. A. Graziewicz, M. J. Longley, R. J. Bienstock, M. Zeviani , W. C. Copeland, "Structure-function defects of human mitochondrial DNA polymerase in autosomal dominant progressive external ophthalmoplegia", Nature Structural & Molecular Biology, 11, 770 - 776 , August 2004.
R. J. Bienstock and W. C. Copeland, " Molecular Insights into NRTI inhibition and mitochondrial toxicity revealed from a structural model of the human mitochondrial DNA polymerase", HIV Anti-retroviral Therapy, and Mitochondria, Clinical and Basic Science Issues , Mitochondrion, Mariana Gerschenson, PhD, Special Issue Editor, Vol 4/2-3, 203-213,July 2004.
S. Dubaele, L. Proiette de Santis, R. J. Bienstock, A. Keriel, M. Stefanini, B. Van Houten, J. M. Egly, “Structure-Function Studies of XPD: Basal Transcription Defect Discriminates Between Xeroderma Pigmentosum and Trichothiodystrophy resulting from Mutations in the XPD Gene”, Molecular Cell, 11, 1-20, June 2003.
M.R. Yudt, C. M. Jewell, R. J. Bienstock and J.A. Cidlowski, “Molecular Orignis for the dominant Negative Functionof Human Glucocorticoid Receptor Beta ”, Molecular and Cellular Biology, 23(12), 4319-4330, June 2003.
R. J. Bienstock, M. Skorvaga, B. S. Mandavilli, and B. Van Houten, “Structural and Functional Characterization of the Human DNA Repair Helicase XPD by Comparative Molecular Modeling and Site-Directed Mutagenesis of the Bacterial Repair Protein UvrB”, J. Biol. Chem., 278 (7), 5309-5316, February 14, 2003.
D. Dai, J. Tang, R. Rose, E. Hodgson, R. J. Bienstock, H. Mohrenweiser, J. A. Goldstein “Identification of Variants of CYP3A4 and Characterization of Their Abilities to Metabolize Testosterone and Chlorpyrifos”, The Journal of Pharmacology and Experimental Therapeutics, 299 (3), 825-831, December 2001.
R. J. Bienstock and J. Carl Barrett, “KAI1, A Prostate Metastasis Suppressor: Prediction of Solvated Structure and Interactions with Binding Partners; Integrins, Cadherins and Cell Surface Receptor Proteins”, Molecular Carcinogenesis, 32 (3), 139-153, November 2001.
R.J. Bienstock, and B. Van Houten, “Molecular Modeling of the Human DNA Repair Protein and Helicase, XPD (xeroderma pigmentosum group D)”, in Proceedings of The Atlantic Symposium on Computational Biology, Genome Information Systems and Techonology, Association for Intelligent Machinery, Inc., (ISBN 0-9707890-0-9) pps. 70-75, 2001.
X. Zhu, C. Borchers, R. J. Bienstock and K.B. Tomer, “Mass Spectrometric Characterization of the Glycosylation Pattern of HIV-gp120 Expressed in CHO Cells”, Biochemistry, 39, 11194-11204,2000.
E.O. Hochleitner, C. Borchers, C.Parker, R. J. Bienstock, and K.B. Tomer, "Characterization of a Discontinuous Epitope of the Human Immunodeficiency Virus (HIV) Core Protein p24 by Epitope Excision and Differential Chemical Modification followed by Mass Spectrometric Peptide Mapping Analysis", Protein Science, 9, 487-497, 2000.
|