Publication Details
Comparison of Normal and Breast Cancer Cell lines using Proteome, Genome and Interactome data.Citation
Patwardhan AJ, EF Strittmatter, DG Camp, II, RD Smith, and M Pallavicini.2005."Comparison of Normal and Breast Cancer Cell lines using Proteome, Genome and Interactome data."Journal of Proteome Research 4(6):1952-1960.
Abstract
Normal and cancer cell line proteomes were profiled using high throughput mass spectrometry techniques. Application of both protein-level and peptide-level sample fractionation combined with LC-MS/MS analysis enabled the confident identification of 2,235 unmodified proteins representing a broad range of functional and compartmental classes. An iterative multi-step search strategy was used to identify post-translational modifications and detected several proteins that are preferentially modified in cancer cells. Information regarding both unmodified and modified protein forms was combined with publicly available gene expression and protein-protein interaction data. The resulting integrated dataset revealed several functionally related proteins that are differentially regulated between normal and cancer cell lines.
