Selected Publications

Inositol Signaling Group

1. Yang L, Reece JM, Cho J, Bortner CD, Shears SB. The nucleolus exhibits an osmotically regulated gatekeeping activity that controls the spatial dynamics and fundations of nucleolin. The Journal of biological chemistry 2008 283(17):11823-11831. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18299322)
2. Brehm MA, Schenk TMH, Zhou X, Fanick W, Lin H, Windhorst S, Nalaskowski MM, Kobras M, Shears SB, Mayr GW. Intracellular localization of human Inositol 1,3,4,5,6-pentakisphosphate 2-kinase. The Biochemical journal 2007 408(3):335-345. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17705785)
3. Choi JH, Williams J, Cho J, Falck JR, Shears SB. Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress. The Journal of biological chemistry 2007 42: 30763-30775. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17702752)
4. Cho, J., Choi, K., Darden, T., Reynolds, P.R., Petitte, J.N., Shears, S.B. (2006) Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's "phosphate crisis”. J Biotechnol. 126: 248-59. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16759730&query_hl=1&itool=pubmed_docsum)
5. Riley, A.M., Trusselle, M., Kuad, P., Borkovec, M., Cho, J., Choi, K., Qian, X., Shears, S.B., Spiess, B., Potter, B.V. (2006) Scyllo-inositol pentakisphosphate as an analogue of myo-inositol 1,3,4,5,6-pentakisphosphate: chemical synthesis, physicochemistry and biological applications. Chembiochem. 7: 1114-22. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16755629&query_hl=1&itool=pubmed_docsum)
6. Pathogenicity of Salmonella: SopE-Mediated Membrane Ruffling is Independent of Inositol Phosphate Signals. Deleu, S., Reece, J.M. and Shears, S.B. (2006) FEBS Lett. Vol 580 pp 1709-1715 [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16500648&query_hl=1&itool=pubmed_docsum)
7. Yang, L., Reece, J., Gabriel, S.E., Shears, S.B. (2006) Apical localization of ITPK1 enhances its ability to be a modifier gene product in a murine tracheal cell model of cystic fibrosis. J. Cell. Sci. vol 119 pp1320-1328 [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16537650&query_hl=1&itool=pubmed_docsum) >
8. Riley, A.M., Deleu, S., Qian, X., Mitchell, J., Chung, S.-K., Adelt, S., Vogel, G., Potter, B.V.L., Shears, S.B. (2006) On the contribution of stereochemistry to human ITPK1 specificity; Ins(1,4,5,6)P4 is not a physiologic substrate. FEBS Letters 580 324-330. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16376887&query_hl=7&itool=pubmed_docsum)
9. Deleu, S., Choi, K., Pesesse, X., Cho, J., Sulis, M.L., Parsons, R., Shears, S.B. (2006) Physiologically-relevant levels of PTEN control the size of the cellular Ins(1,3,4,5,6)P5 pool. Cellular Signalling 18 488-498. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15979280&query_hl=7&itool=pubmed_docsum)
10. Shears, S.B. (2005) Can intervention in inositol phosphate signalling pathways improve therapy for cystic fibrosis? Expert Opin Ther Targets. 9 1307-1317. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16300477&query_hl=7&itool=pubmed_docsum)
11. Shears, S.B. (2005) Rounding up the usual suspects: protein kinases as therapeutic targets. Drug Discov Today 10 240-242. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15708741&query_hl=7&itool=pubmed_docsum)
12. Shears, S.B. (2005) Telomere maintenance by intracellular signals: new kid on the block? Proc Natl Acad Sci U S A. 102 1811-1812. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15684046&query_hl=1&itool=pubmed_docsum)
13. Ribeiro, C.M., Paradiso, A.M., Carew, M.A., Shears, S.B., Boucher, R.C. (2005) Cystic fibrosis airway epithelial Ca2+ signaling. The mechanism for the larger agonist-mediated Ca2+ signals in human cystic fibrosis airway epithelia. J. Biol. Chem. 260, 10202-10209. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15647273&query_hl=3&itool=pubmed_docsum)
14. Choi, K., Mollapour-Bever, E. and Shears, S.B. (2005) Signal transduction during environmental stress: InsP8 operates within highly restricted contexts. Cellular Signalling. 17, 1533-1541. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15936174&query_hl=5&itool=pubmed_docsum)
15. Qian, X., Mitchell, J.J., Wei, S.J., Williams, J., Petrovich, R.M., and Shears, S.B. (2005) The Ins(1,3,4)P3 5/6-kinase/Ins(3,4,5,6)P4 1-kinase is not a protein kinase. Biochem. J. 389, 389-395. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15762844&query_hl=7&itool=pubmed_docsum)
16. Pesesse, X., Choi, K., Zhang, T. and Shears, S.B. (2004) Signaling by higher inositol polyphosphates. Synthesis of bisdiphosphoinositol tetrakisphosphate ("InsP8") is selectively activated by hyperosmotic stress. J. Biol. Chem. Accelerated Communication 279, 43378-43381. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15316027&query_hl=1&itool=pubmed_docsum)
17. Chu,C., Alapat, D., Wen, X., Timo, K., Burstein, D., Lisanti, M., Shears, S. B., Kohtz, D. S. (2004) Ectopic expression of murine diphosphoinositol polyphosphate phosphohydrolase 1 attenuates signaling through the ERK1/2 pathway. Cellular Signalling 16 1045-1059 [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15212765)
18. Shears, S.B. (2004) How versatile are inositol phosphate kinases? Biochem. J. 377 265-280. [Abstract] (http://www.biochemj.org/bj/imps/refer.htm?MSID=BJ20031428.htm)
19. Hidaka, K., Kanematsu, T., Caffrey, J.J., Takeuchi, H., Shears, S.B., Hirata, H. (2003) The Importance to chondrocyte differentiation of changes in expression of the Multiple Inositol Polyphosphate Phosphatase. Exp. Cell Res. 290 254-264. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14567985&dopt=Abstract)
20. Yu J., Leibiger B., Yang S.N., Caffery J.J., Shears S.B., Leibiger I.B., Barker C.J,. Berggren P.O. (2003) Cytosolic multiple inositol polyphosphate phosphatase in the regulation of cytoplasmic free Ca2+ concentration. J. Biol. Chem. 278 46210-46218. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12963730&dopt=Abstract)
21. Hua, L.V., Hidaka, K., Zhang, Pesesse, X., Barnes, L.D. Shears, S.B. (2003) Paralogous murine Nudt10 and Nudt11 genes have differential expression patterns but encode identical proteins that are physiologically competent diphosphoinositol polyphosphate phosphohydrolases. Biochem. J. 373 81-89 [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12689335&dopt=Abstract)
22. Ho, M.W.Y., and Shears, S.B. (2002) Regulation of calcium-activated chloride channels by inositol 3,4,5,6-tetrakisphosphate. Current Topics in Membranes 53, 345-363.
23. Hidaka K, Caffrey JJ, Hua L, Zhang T, Falck JR, Nickel GC, Carrel L, Barnes LD, Shears SB. (2002) An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase. J. Biol. Chem. 277, 32730-32738. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12105228&dopt=Abstract)
24. Renstrom E, Ivarsson R, Shears SB. (2002) Inositol 3,4,5,6-tetrakisphosphate inhibits insulin granule acidification and fusogenic potential. J. Biol. Chem. 277, 26717-26720. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12055181&dopt=Abstract)
25. Dubois,E., Scherens B, Vierendeels F., Ho M.W.Y., Messenguy F., Shears S.B. (2002) In Saccharomyces cerevisiae, the inositol polyphosphate kinase activity of Kcs1p is required for resistance to salt stress, cell wall integrity, and vacuolar morphogenesis. J.Biol.Chem. 277, 23755-23763. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11956213&dopt=Abstract)
26. Ho, M.W.Y., Yang, X., Carew, M.A., Zhang, T., Hua, L., Kwon, Y-U., Chung, S.K., Adelt, S., Vogel, G., Riley, A.M., Potter, B.V.L., Shears, S.B. (2002) Regulation of Ins(3,4,5,6)P4 Signaling by a Reversible Kinase/Phosphatase. Current Biology 12, 477-482. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11909533&dopt=Abstract)
27. Adelt S, Plettenburg O, Dallmann G, Ritter FP, Shears SB, Altenbach H, Vogel G. (2001) Regiospecific phosphohydrolases from Dictyostelium as tools for the chemoenzymatic synthesis of the enantiomers D-myo-inositol 1,2,4-trisphosphate and D-myo-inositol 2,3,6-trisphosphate: non-physiological, potential analogues of biologically active D-myo-inositol 1,3,4-trisphosphate. Bioorg. Med. Chem. Lett. 11, 2705-2708. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11591506&dopt=Abstract)
28. Liu C, Riley AM, Yang X, Shears SB, Potter BV. (2001) Synthesis and biological activity of D- and L-chiro-inositol 2,3,4,5-tetrakisphosphate: design of a novel and potent inhibitor of Ins(3,4,5,6)P4 1-kinase/Ins(1,3,4)P3 5/6-kinase. J Med Chem. 44 2984-2989. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11520207&dopt=Abstract)
29. Abel K, Anderson RA, Shears SB. (2001) Phosphatidylinositol and inositol phosphate metabolism. J. Cell Sci. 114, 2207-2208. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11493657&dopt=Abstract)
30. Kreda SM, Sumner M, Fillo S, Ribeiro CM, Luo GX, Xie W, Daniel KW, Shears S, Collins S, Wetsel WC. (2001) alpha(1)-Adrenergic Receptors Mediate LH-Releasing Hormone Secretion through Phospholipases C and A(2) in Immortalized Hypothalamic Neurons. Endocrinology 142, 4839-4851. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11606452&dopt=Abstract)
31. Ho, M.W.Y., Kaetzel, M.A., Armstrong, D.L. and Shears, S.B. (2001) Regulation of a Human Chloride Channel: A Paradigm for Integrating Input from Calcium, CaMKII and Ins(3,4,5,6)P4. J. Biol. Chem. 276, 18673-18680. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11279175&dopt=Abstract)
32. Caffrey, J.J., Darden, T., Wenk, M.R. and Shears, S.B. (2001) Expanding Coincident Signaling by PTEN through its Inositol 1,3,4,5,6-Pentakisphosphate 3-phosphatase Activity. FEBS Lett. 499, 6-10. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11418101&dopt=Abstract)
33. Caffrey, J.J. and Shears, S.B. (2001) Genetic rationale for microheterogeneity of human diphosphoinositol polyphosphate phosphohydrolase type 2. Gene 269, 53-60. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11376937&dopt=Abstract)
34. Zhang, T., Caffrey, J.J. and Shears, S.B. (2001) The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity. FEBS Lett. 494, 208-212. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11311242&dopt=Abstract)
35. Shears, S.B. (2001) Assessing the omnipotence of inositol hexakisphosphate. Cellular Signalling 13, 151-158. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11282453&dopt=Abstract)
36. Zhou, D. Chen, L.-M. Hernandez, L., Shears, S.B. and Galán, J.E. (2001) A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Molecular Microbiology 39, 248-259. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11136447&dopt=Abstract)
37. Dewaste, V., Pouillon, V., Moreau, C., Shears, S.B., Takazawa, K., Erneux, C. (2000) Cloning and expression of a cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase C. Biochem. J. 352, 343-351. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11085927&dopt=Abstract)
38. Yang, X. and Shears, S.B. (2000) Multitasking in Signal Transduction by a Promiscuous Human Ins(3,4,5,6)P4 1-Kinase/Ins(1,3,4)P3 5/6-Kinase. Biochem. J. 351, 551-555. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11042108&dopt=Abstract)
39. Chi, H., Yang, X., Kingsley, P.D., O'Keefe, R.J., Puzas, J.E., Rosier, R.N., Shears, S.B. and Reynolds, P.R. (2000) Targeted Deletion of Minpp1 Provides New Insight into the Activity of Multiple Inositol Polyphosphate Phosphatase. In Vivo. Mol. Cell. Biol. 20, 6496-6507. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10938126&dopt=Abstract)
40. Carew, M.A., Yang, X. Schultz, C. and Shears, S.B. (2000)
    Ins(3,4,5,6)P4 inhibits an apical calcium-activated chloride conductance in polarized monolayers of a cystic fibrosis cell-line. J. Biol. Chem. 275, 26906-26913. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10842174&dopt=Abstract)
41. Shears, S.B. (2000) Transcriptional Regulation: A new dominion for inositol phosphate signaling? Bioessays 22, 786-789. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10944580&dopt=Abstract)
42. Saiardi, A., Caffrey, J.J., Snyder, S. H. and Shears, S. B. (2000) The Inositol Hexakisphosphate Kinase Family: Catalytic Flexibility, and Function in Yeast Vacuole Biogenesis. J. Biol. Chem. 275, 24686-24692. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10827188&dopt=Abstract)
43. Caffrey, J.J., Safrany, S.T., Yang, X., and Shears, S.B. (2000) Discovery of Molecular and Catalytic Diversity Among Human Diphosphoinositol Polyphosphate Phosphohydrolases: An Expanding NUDT Family. J. Biol. Chem. 275, 12730-12736. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10777568&dopt=Abstract)
44. Saiardi, A., Caffrey, J.J., Snyder, S. H. and Shears, S. B. (2000) Inositol Polyphosphate Multikinase (ArgRIII) Determines Nuclear mRNA Export in Saccharomyces cerevisiae. FEBS Lett. 468, 28-32. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10683435&dopt=Abstract)
45. Stiles AR, Qian X, Shears SB, Grabau EA. (2008) Metabolic and signaling properties of an Itpk gene family in Glycine max. FEBS Lett.582, 1853-1858. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18474240)
46. Choi K , Mollapour E, Choi JH, Shears SB (2008) Cellular energetic status supervises the synthesis of bis-diphosphoinositol tetrakisphosphate independently of AMP-activated protein kinase. Molecular pharmacology 74(2):527-536. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18460607)
47. Cho J, King J, Qian X, Harwood AJ, Shears SB. (2008) Dephosphorylation of 2,3-bisphosphoglycerate by MIPP expands the regulatory capacity of the Rapoport-Luebering glycolytic shunt. Proceedings of the National Academy of Sciences of the United States of America 105(16):5998-6003. [Abstract] (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18413611)

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