Plantier JC, Gueudin M, Damond F, Lemee V, Simon F; IAS Conference on HIV Pathogenesis and Treatment (2nd : 2003 : Paris, France).
Antivir Ther. 2003; 8 (Suppl.1): abstract no. 265.
Unite de Virologie, CHU Rouen, GRAM, IFR23, Rouen, France
BACKGROUND: Discordance between quantitative assays and clinical-immunological status or persistant undetermined serological profiles were reported in African patients or their relatives living in France, HIV-2 , HIV-1 group O or N infections being excluded. OBJECTIVES: Virological characterization of the 'HIV-U' , U for HIV unknown. To be classified as belonging to 'HIV-U', patients must be naive of anti-retroviral treatment with at least four of the following criteria: EIA commercial assays positive or discordance between EIA assays; Western-Blot complete or primary infection profile without evolution during 3 months of following or atypical profile with anti-Pol antibodies; PCR H-Pol integrase positive or negative; viral load discordance with clinical status or asymptomatic undetectable and negative PCRs in highly conserved pol gene. Isolation from PBMC, peptide-based-EIA antibodies characterization and nested and real-time PCR algorithm were processed. RESULTS: Ten documented cases with a long follow-up (>12 months), all originating from Central or West Africa were classified as HIV-U. PBMC cultures remained negative. Proviral DNA, when sucessfully amplified (n=3) and peptide-based serology (n=10) favoured HIV-1 group M apparte-nance, with no or low replication. All these strains were related to CRF and particularly CRF02_AG or G-like associated form. CONCLUSION: CRF02_AG or G-like are increasingly imported in Europe. These atypical infections involving recombinant related forms plaid fo further clinical and molecular investigations to explain this low level of replication and to reinforce the diagnosis capacity particularly in blood banking.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Africa
- Europe
- France
- Genes, pol
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Humans
- Natural History
- Polymerase Chain Reaction
- Viral Load
- genetics
Other ID:
UI: 102262549
From Meeting Abstracts