Edelman AS, Zolla-Pazner S; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 579 (abstract no. T.C.P.74).
NYU Med. Ctr. and V.A. Hospital NY, NY, USA
OBJECTIVE: To elucidate the immunoregulatory mechanisms involved in B lymphocyte responses of cells from HIV-seropositive (HIV+) subjects. METHODS: Proliferative responses of mononuclear cells (MNC) to B cell mitogens, (anti-u and Staphylococcus aureus Cowan I [SAC]) were determined by 3H-thymidine incorporation in 3 or 4 day cultures. Mixed lymphocyte culture supernatant (MLC-sup) and indomethacin were added to certain cultures. RESULTS: Average proliferative responses of MNC from HIV+ patients to each mitogen are significantly defective (25-35% of control [HIV-] responses). Addition of MLC-sup increases responses of both HIV+ and HIV- MNC to anti-u (45% and 90% respectively) and SAC (60% in both groups). Addition of indomethacin increases responses to SAC, (60-70% increase for both groups). Effects of MLC-sup and indomethacin are additive. To determine if deficient responses were influenced by the altered frequency of lymphocyte subsets in the HIV+ cultures, correlations were sought between proliferation and percent of lymphocyte subsets. Proliferative responses to anti-u and SAC in the presence of both MLC-sup and indomethacin are correlated positively with the percent of B and CD4+ T cells, and negatively with the percent of CD8+ T cells in culture. Mathematical correction of the data for the abnormal percentages of B, CD4+ T and CD8+ T cells results in responses that are comparable to those of control cells. CONCLUSION: Deficient responses may be due to quantitative differences in the responding B cells and regulatory pathways mediated by CD4+ and CD8+ T cells and not to qualitative differences in the ability of the patients' B cells to respond to these mitogens.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- Antigens, CD8
- B-Lymphocytes
- Growth Substances
- HIV Infections
- HIV Seropositivity
- Humans
- Lymphocyte Culture Test, Mixed
- Lymphocyte Subsets
- Mitogens
- Pokeweed Mitogens
- T-Lymphocytes
- immunology
Other ID:
UI: 102178933
From Meeting Abstracts