Lafrere JJ, Mariotti M, Poste Y, Vittecoq D; International Conference on AIDS.

Int Conf AIDS. 1991 Jun 16-21; 7: 215 (abstract no. W.B.2132).

Institut National de Transfusion Sanguine, Paris, France

OBJECTIVE: To appreciate the efficacy of plasma rich in anti-p24 antibodies (HIV+P) in AIDS patients through a quantitative PCR assay measuring the number of viral copies (VC). METHODS: 5 AIDS patients receiving every 14 days (d) 7 times an infusion of HIV+P and 3 AIDS patients receiving at the same rythm seronegative plasma (HIV-P) (negative controls) were studied through PCR before the 1st infusion (d.1), during the transfusional period (d.57) and after the last infusion (d.100 and 200). The number of VC was established in 100 000 cells through a quantitative PCR. Using our conditions, PCR detected 1 VC in 100 000 cells. RESULTS: The figure shows the mean number of VC over time in HIV+P (bold) and HIV-P (stippled) groups. No significant decrease in the number of VC over time was observed in individuals of both groups (Spearman test). TABULAR DATA, SEE ABSTRACT VOLUME. DISCUSSION AND CONCLUSIONS: Our results did not suggest an effect of passive immunotherapy (PA) on the number of VC in AIDS patients and did not confort the results of Karpas (PNAS USA 1990;87:7613). However, we measured the DNA integrated in cells and not the serum RNA. Determination of RNA quantity would be more accurate to appreciate the efficacy of PA on viral replication in AIDS.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• HIV
• HIV Antibodies
• HIV Core Protein p24
• HIV Infections
• HIV Seronegativity
• HIV Seropositivity
• Humans
• Immunization, Passive
• Polymerase Chain Reaction
• immunology
• virology

Other ID:

• 3213291

UI: 102192736

From Meeting Abstracts