IEVEN M, LOENS K, URSI D, SENTERRE JM, VAN AUTGAERDEN T, GERARD E, GRANDJEAN JL, GOOSSENS H; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. D-2015.

Center for Molecular Diagnostics, University Hospital Antwerp, Edegem, Belgium.

BACKGROUND: A national QC study was organized in Belgium to assess the ability of laboratories to detect HSV and VZV in cerebrospinal fluid (CSF). METHODS: The QC panels for HSV consisted of 3 virus negative CSF samples and 6 samples spiked with HSV1 at concentrations of 5.10[0.9] TCID[50] (n=2) and 5.10[2.9] TCID[50] (n=3) and one mixture of HSV1 and VZV. The VZV panel consisted of 1 virus negative control, 3 CSF spiked with HSV 1 or 2 and CMV for specificity testing, 6 samples of CSF spiked with VZV 100 copies/0.001 ml(n=1), VZV 15 copies/0.001 ml(n=1), VZV 10 copies/0.001 ml(n=2), VZV mixed with HSV1 (n=1). 12 laboratories participated for HSV and 9 for VZV detection. RESULTS: All participants applied their own techniques for sample preparation and amplification. To avoid contamination most labs worked into separate rooms and used dUTP/UDG. Inhibition control was included in 5/12 labs for HSV detection and in 6/9 labs for VZV detection. For HSV1 no false positive results were recorded. For the lowest concentration of HSV1 21/24 (87.5%) and for the highest concentrations of HSV1 36/36 (100%) of correct results were recorded. The mixture HSV1/VZV was also detected by all labs. For VZV, there were no false positive results and no cross reactions with non-VZV samples. All participants detected VZV in concentrations up to 10 copies/0.001 ml and for 10/18 (55.5%) of the samples with a VZV concentration of 1 copy/0.001 ml correct results were recorded. One lab applying a multiplex PCR detected only HSV in the mixture of VZV/HSV1. CONCLUSIONS: Despite the great diversity of protocols applied, results were very satisfactory.

Publication Types:

• Meeting Abstracts

Keywords:

• Belgium
• Hematologic Tests
• Herpes Simplex
• Herpesvirus 1, Human
• Herpesvirus 3, Human
• Laboratories
• Polymerase Chain Reaction
• Quality Control
• Sensitivity and Specificity
• Simplexvirus
• methods

Other ID:

• GWAIDS0027844

UI: 102267468

From Meeting Abstracts