Velema JP, Klokke AH, Kigadye FC, Dolmans WM, Shao JF, van Asten HA, Heessen FW; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: C356 (abstract no. PoC 4680).
Nijmegen University, The Netherlands.
OBJECTIVES: Evaluation of performance of screening by anti-HIV EIA for prevention of transmission of HIV by blood transfusion in peripheral hospitals in Mwanza region, Tanzania. METHODS: Organon Vironostika anti-HTLV-III EIA was introduced in 6 peripheral hospital laboratories. Quality control (QC) was performed at two levels. Samples were retested in Bugando Medical Centre (BMC), the reference laboratory for northern Tanzania (Lake Zone). Wellcozyme or Organon EIA was used for all positive and doubtful samples and 10% of negatives at BMC and samples were shipped to the Netherlands for further evaluation with Abbott recombinant HIV-1/HIV-2 EIA and, if reactive, with Western Blot. RESULTS: QC at BMC showed sensitivity in the peripheral hospitals to be 93.5% and specificity 90.0%. When QC was--done in The Netherlands sensitivity was 91.4% and specificity was 90.5%. Of 1514 samples evaluated by QC, 62 had an indeterminate WB by CDC criteria; 50 of these were negative by Organon EIA and 49 showed only p17, p24 or p55 bands. Gag-only patterns are found frequently in African sera and it is not clear if these samples from high risk areas can be considered as safe for transfusion. CONCLUSIONS: We found a rather good performance of EIA for HIV-screening in peripheral hospitals. Assuming a 10% prevalence of HIV, from each 1.000 units of blood 100 are contaminated and more than 90 cases of HIV-transmission can be prevented using EIA for screening.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Blotting, Western
- Equine Infectious Anemia
- Evaluation Studies
- HIV Infections
- HIV Seropositivity
- HIV-1
- HIV-2
- Immunoenzyme Techniques
- Mass Screening
- Netherlands
- Quality Control
- Tanzania
- blood
Other ID:
UI: 102200065
From Meeting Abstracts