Costa L, Janini M, Pinto M, Diaz R; International Conference on AIDS.

Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. MoPpA1005.

L. Costa, UNIFESP - EPM, Laboratorio de Retrovirologia, R. Pedro de Toledo 781/16andar, Vila Clementino - Sao Paulo, SP, CEP:04032-000, Brazil, Tel.: +55-11-570 82 26 or 5712130, Fax: +55-11-571 21 30, E-mail: ljcosta@hotmail.com

In a previous work a subtype B and C HIV-1 dual infection in a husband, his wife, and their child was detected. The simultaneous presence of highly similar sets of B and C HIV-1 infecting the 3 family members, confirmed by DNA sequence analysis of pol, gag, and env, together with available epidemiologic information, may indicate that the husband was the source of dual infections. To follow up the quasispecies analyses in order to identify any change in subtype patterns previously described. Blood samples from the 3 members were taken at the first time of infection and three years later. Samples were used to DNA extraction. Clones of each sample at each time point, spanning the env gene, were generated by PCR end-point dilution, sequenced and used to characterized HIV-1 subtypes. Our results demonstrated that although sequences from subtype B and C were equally represented at the first time point in all family members, at the last time point subtype B sequences were barely found: 2 clones out of 13; 3 out of 10; and 1 out of 9 in father, mother and child, respectively. We also found sequences belonging to subtype F in the last time point sample from the father (4 clones). Contamination was ruled out by comparing these subtype F sequences with that studied in our Lab. We were not able to detected subtype F in mother or child samples. Data may suggest an ongoing competition between B and C subtypes with the C viruses prevailing. Since it seems to occur in all family members, an advantage to the subtype C viruses might be proposed. The presence of subtype F in the father sequences may indicate the establishment of a new infection process despite the previous dual infection event. The husband's high-risk sexual behavior may corroborate this hypothesis. This is the first report of a triple HIV-1 infection with different subtypes.

Publication Types:

• Meeting Abstracts

Keywords:

• Base Sequence
• Child
• Cluster Analysis
• Genes, env
• Genes, gag
• Genes, pol
• HIV-1
• Humans
• Polymerase Chain Reaction
• Sequence Analysis, DNA
• genetics
• reverse transcriptase, Human immunodeficiency virus 1

Other ID:

• GWAIDS0000041

UI: 102237530

From Meeting Abstracts