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Core FacilitiesThe NIEHS DNA Sequencing Core uses biochemical methods to determine the order of nucleotide bases—adenine, guanine, cytosine and thymine—in a DNA sample. The core uses Perkin-Elmer ABI Model 3100 and Model 377 sequencers which offer a variety of services including single pass primer extension using plasmid DNA, PCR products or genomic DNA as a sequencing template. The single pass extension using conventional sequencing primers such as M13, T7, T3 and SP6 or a custom primer, can provide 600-650 base pairs of "good" or viable sequence data with a quick turnaround time. Shotgun sequencing strategies can be used to sequence a variety of samples including cosmids, BACs and PACs. The DNA Sequencing Core does not accept samples from the general public, but any investigator from within the Institute may submit samples for analysis at no cost. Greg Solomon manages the facility, with biologist Hannah Saunders and sequencing engineer John Otstot assisting in the generation of sequence data. |
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