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August 19, 2005

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Detection of Cereal Proteins and DNA Using MS, ELISA, and PCR

[Transcript of Dr. Hurkman's remarks]

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Author: William J. Hurkman, Ph. D., USDA/ARS

Table of Contents
  1. Detection of Cereal Proteins and DNA Using MS, ELISA, and PCR
  2. Wheat Flour Proteins
  3. Gliadin and Glutenins Are Prolamins
  4. Detection Methods
  5. MS Protein Identification
  6. Wheat Endosperm Proteins
  7. MS Protein Identification
  8. ELISA
  9. Monoclonal Antibody Production
  10. Sandwich ELISA
  1. R5 Monoclonal Antibody
  2. R5 Monoclonal Antibody Recognizes QQPFP
  3. R5 ELISA Pros
  4. R5 ELISA Cons
  5. PCR Amplification
  6. PCR Primers for Prolamins
  7. PCR Primers for Prolamins
  8. PCR Specificity
  9. PCR
  10. Summary

Public Meeting On: Gluten-Free Food Labeling August 19, 2005


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Hypertext updated by dav November 14, 2005