Purpose: These tools apply Shannon Entropy as a measure of variation in DNA and protein sequence alignments. ENTROPY-TWO compares two sets of aligned sequences (named query and background sequences), and determine if there is greater variability in one set relative to the other. Each position with a significant difference in variability between these two sets will be highlighted against a query consensus.
Details: Entropy has the option of randomizing the combined sequence sets either with replacement or no replacement, and recalculating the entropies for two random data sets broken down into sets of the same size as the original two.If you wish to include sequences of variable length in the alignment, add asterisks (*) to compensate for shorter sequences. Avoid including columns that are all stars. For more details, see explanation of Entropy Options.
