N-GlycoSite

Purpose: This tool highlights and tallies N-Linked Glycosylation site (Nx[ST] patterns, where x can be any amino acid) in an amino acid alignment. If you just want to tally the number of N-glycosylation sites, protein sequences do not need to be aligned.

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Details:
An N-linked glycosylation site pattern, NX[ST] (where X can be any amino acid), is called a sequon. This pattern forms the basis for most of the analyses on this web site. The extent of N-linked glycosylation of a particular N-linked glycosylation site, however, can be influenced by the content in which it is embedded, and could be expanded to a 4-amino acid NX[ST]Y pattern, where the amino acid in the X or Y position of NX[ST]Y pattern can be important determinants of N-linked glycosylation efficiency. For example, a proline in position X or Y strongly disfavors N-linked glycosylation. Thus we provide NX[ST] or NX[ST]Y summaries.

O-linked glycosylation signals are more difficult predict in protein sequences than N-linked sites, but one can estimate their positions in sequences using the NetPhos program (at Center for Biological Sequence Analysis).

References:

1. Zhang M et al., Glycobiology. 14(12):1229-46 (2004) -- please cite this reference if you use our tool in a publication.
2. Marshall RD, Biochem Soc Symp. 40:17-26 (1974)
3. Kasturi et al., Biochem J. 323 (Pt 2):415-9 (1997)
4. Mellquist JL et al., Biochemistry. 37(19):6833-7 (1998)