Microscope: Scanning Probe - Dynamic Force
The Asylum MFP-3D BIO is a versatile atomic force microscope (AFM) that combines molecular resolution imaging and picoNewton force-based measurements on an inverted optical microscope. It is designed specifically for biological applications and can be used to:
- Study individual protein or DNA molecules in situ
- Image the surface of living cells
- Measure the adhesive forces of proteins on cell surfaces
- Conduct single-molecule force spectroscopy of single DNA, protein, and polysaccharide molecules
- Conduct nanolithography and manipulation of samples on the nanometer and picoNewton scale.
With the MFP-3D-BIO, researchers can locate sample features using brightfield, Zernike phase contrast, or fluorescence microscopy, then zoom into the nanometer scale using the AFM. Powerful optical microscopy techniques can be combined with the AFM, including confocal, fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), and total internal reflection fluorescence (TIRF). At EMSL, we are in the process of adding the capability to synchronize with confocal measurements to our MFP-3D-BIO. Advanced software capabilities allow the researcher to display results intuitively in three dimensions, then use powerful analysis and scripting tools to extract and graph quantitative information.
All Related Publications Related Publications
- In Vitro Evolution of a Peptide with a Hematite Binding Motif That May Constitute a Natural Metal-Oxide Binding Archetype.
- Computer Simulation of Uranyl Uptake by the Rough Lipopolysaccharide Membrane of Pseudomonas aeruginosa.
- Binding and Direct Electrochemistry of OmcA, an Outer-Membrane Cytochrome from an Iron Reducing Bacterium, with Oxide Electrodes: A Candidate Biofuel Cell System.
- Correlation between fundamental binding forces and clinical prognosis of Staphylococcus aureus infections of medical implants.
- Specific Bonds between an Iron Oxide Surface and Outer Membrane Cytochromes MtrC and OmcA from Shewanella oneidensis MR-1.
