Microscope: Fluorescence - Single-Molecule
The single-molecule optical microscope is designed to study complex reaction dynamics such as enzymatic reactions, protein-protein interactions, and interfacial electron transfer process. It is designed and adapted specifically for single-molecule spectroscopy experiments.
Constructed by PNNL researchers, this instrument comprises an inverted optical microscope, a variety of continuous-wave and pulsed lasers, optics, spectrometers, and ultrasensitive photon detectors. Researchers use this instrument to study a variety of samples in solid phase and solution. For solid-phase experiments, the diluted species (< 1 nM) are spin-coated on a microscopy glass cover slip with a surface coverage of about 0.1 molecule/μm2. For solution experiments, the species can be covalently linked to the modified glass surface or immobilized by agarose gel.
Users with extensive experience in optical microscopy, optics, and photochemistry can be trained to use this instrument as needed for their research.
All Related Publications Related Publications
- Combined Spectroscopic and Topographic Characterization of Nanoscale Domains and Their Distributions of a Redox Protein on Bacterial Cell Surfaces .
- Tip-Enhanced Near-Field Raman Spectroscopy Probing Single Dye-Sensitized TiO2 Nanoparticles.
- Revealing Two-State Protein-Protein Interaction of Calmodulin by Single-Molecule Spectroscopy.
- Single-Molecule Dynamics Reveals Cooperative Binding-Folding in Protein Recognition .
- Site-Specific Raman Spectroscopy and Chemical Dynamics of Nanoscale Interstitial Systems.
