Spectrometer: Fluorimeter
Quick Specs
- Capable of measuring excitations and emission spectra of solids and solutions
- Equipped with single photon counting ability
- Maximum efficiency ranging from 200 nm to 700 nm
EMSL's SPEX Fluorolog 2 is a high-sensitivity fluorescence spectrometer used for measuring excitation and emission spectra of solids and solutions. The instrument is equipped with single photon counting capabilities in the ultraviolet and visible, with maximum instrument efficiency ranging from 200 nm to 700 nm. Fluorescence detection can be extended into the infrared (0.8 μm to 1.7 μm) with use of a liquid nitrogen cooled germanium detector.
All work with this instrument and in EMSL labs must be performed in compliance with EMSL practices and permits.
The instrument's built-in light source consists of a 450-W Xe lamp with a quartz envelope for strong ultraviolet and visible wavelengths. Average luminance is 35,000 cd/cm2. Other light sources available for excitation include a large-frame Argon ion laser, a Nd:YAG laser, and a conventional mercury lamp.
The spectrometer is equipped with dual grating monochromometers (f/4, 0.22-m focal length) for maximum stray light rejection in excitation and emission. A single grating monochromometer is available for higher throughput. Manual entrance and exit slits for both monochromometers are continuously variable from 10 μm to 8 mm. A variety of diffraction gratings are available (see chart below) with varying groove densities and blazes. Manual polarizers are available for polarization anisotropy measurements. Single photon counting is effected using a thermoelectrically cooled photomultiplier. Fluctuations in source lamp intensities are monitored by a photocathode for spectral correction.
Performance specifications and sample preparation information are provided below to facilitate user planning.
Sample Preparation and Options
Generally, this instrument can be used to analyze samples of any physical states (solid, liquid, gas) that display fluorescence from the near-ultraviolet to near-infrared range.
Solution samples may be analyzed in either right-angle or front-faced geometries, while solid samples are analyzed mostly in front-face geometry. Manual polarizers are available for polarization anisotropy measurements. A cryogenic workstation is available for analysis of either liquid or solid samples from room temperature to liquid nitrogen or liquid helium temperatures. Users are required to provide their own sample cuvettes.
| Available gratings include: | ||
|---|---|---|
| 150 g/mm | 500-nm blaze | single |
| 300 g/mm | 500-nm blaze | pair |
| 600 g/mm | 125-nm blaze | pair |
| 600 g/mm | 1000-nm blaze | pair |
| 1200 g/mm | 250-nm blaze | pair |
| 1200 g/mm | 330-nm blaze | single |
| 1200 g/mm | 750-nm blaze | pair |
Instrument operation is fairly simple; thus, individuals who have taken spectroscopic courses or training in college usually are permitted unsupervised operation of the instrument after less than a day of hands-on training. However, because of the instrument's high use, users must completely follow the associated operational procedure in order to prevent damage to the hardware or software and reduce equipment downtime.
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