RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lepidocybium(*) flavobrunneum(*)
FDA Seafood List Common Name: Escolar(*)
FDA Seafood List Market Name: Escolar(*)
Unique IEF Gel Code: 19970424gel05
Unique Web Code: ech01Srr
Tissue Aliquot Code: lepiflav L95-001 #15p Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	23	3	0.0605694	4	0.434628	5.9	4.4	4.55	23.4	4	0.0605829
2	5.2	110	14.1	0.0690991	5	0.650174	8.8	6.6	5.2	111.2	5.3	0.0688504
3	5.85	226	29	0.10214	5	0.850189	11.5	8.7	5.85	226.3	4.7	0.101612
4	6.55	370	47.6	0.107801	8	1.38403	18.8	14.1	6.55	368.5	7.9	0.0989916
5	6.85	406	52.2	0.0334089	6	0.313282	4.3	3.2	6.85	407.6	5.8	0.030586
6	7.35	472	60.7	0.0651508	4	0.447229	6.1	4.6	7.35	472.1	4.1	0.0613834
7	8.15	597	76.7	0.0770057	7	0.963689	13.1	9.8	8.15	596.5	7.1	0.0760499
8	8.45	666	85.6	0.108333	6	1.20172	16.3	12.2	8.45	665.2	6.4	0.106255
9	8.65	714	91.8	0.102757	4	0.738028	10	7.5	8.65	713.6	4.3	0.0974313
10	9.3	754	96.9	0.032658	8	0.382119	5.2	3.9	9.3	753.1	7.6	0.02826

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.802	54	7	0.0179257	4	0.126371	0.5	0.5	4.802	53.3	4.4	0.0163182
2	5.577	174	22.4	0.0179902	13	0.387051	1.7	1.5	5.577	180.2	13.2	0.0165622
3	5.784	210	27.1	0.0677723	3	0.383386	1.7	1.5	5.784	209.3	3.2	0.067539
4	6.071	268	34.5	0.0495474	3	0.271642	1.2	1	6.071	268.1	3.2	0.0478923
5	6.181	291	37.5	0.329129	3	1.58667	6.9	6.1	6.181	290.7	2.8	0.324101
6	6.271	310	39.9	0.114451	3	0.557682	2.4	2.1	6.271	309.6	2.9	0.109768
7	6.357	328	42.3	0.100382	4	0.692751	3	2.7	6.357	327.6	4.3	0.0916699
8	6.419	341	43.9	0.0954398	2	0.38101	1.6	1.5	6.419	340.7	2.2	0.0966416
9	6.51	360	46.4	0.086833	3	0.350695	1.5	1.3	6.51	359	2.9	0.0679695
10	6.524	363	46.8	0.063353	3	0.274383	1.2	1.1	6.524	363.4	2.9	0.054095
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.588	373	48.1	0.294277	2	0.994961	4.3	3.8	6.588	372.5	2.1	0.268429
12	6.638	379	48.8	0.0707042	6	0.637109	2.8	2.4	6.638	377.6	6.1	0.0592965
13	6.679	384	49.5	0.269536	2	0.961526	4.2	3.7	6.679	384.2	2.1	0.259966
14	6.729	390	50.3	0.0702081	1	0.197735	0.9	0.8	6.729	390.9	1.4	0.0774752
15	6.796	398	51.3	0.424989	2	1.94105	8.4	7.4	6.796	397.4	2.5	0.439628
16	6.93	415	53.5	0.199522	3	1.02386	4.4	3.9	6.93	415.4	2.9	0.197682
17	7.029	428	55.2	0.111032	7	1.19023	5.1	4.6	7.029	426.6	6.6	0.101577
18	7.106	438	56.4	0.192543	6	1.90732	8.2	7.3	7.106	438.4	5.9	0.182148
19	7.235	455	58.6	0.405995	2	1.7059	7.4	6.5	7.235	455.1	2.3	0.414665
20	7.357	471	60.7	0.0343098	4	0.144361	0.6	0.6	7.357	469.9	3.7	0.0220327
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.558	502	64.7	0.105359	18	3.12722	13.5	12	7.558	507.5	18	0.098021
22	7.805	540	69.6	0.100554	2	0.325906	1.4	1.2	7.805	540.6	2.1	0.086815
23	7.903	555	71.5	0.486601	3	2.49624	10.8	9.6	7.903	554.9	2.9	0.488568
24	8.379	646	83.2	0.0105662	10	0.144378	0.6	0.6	8.379	644.9	10.1	0.00802837
25	8.435	659	84.9	0.0431174	4	0.269526	1.2	1	8.435	658.8	3.7	0.0408551
26	8.481	670	86.3	0.0350185	4	0.248255	1.1	1	8.481	669.1	4	0.0349141
27	8.535	683	88	0.0279777	6	0.288698	1.2	1.1	8.535	682	5.9	0.0275211
28	8.573	692	89.2	0.0244417	5	0.192452	0.8	0.7	8.573	692.4	4.7	0.0231207
29	8.736	716	92.3	0.012121	4	0.10101	0.4	0.4	8.736	716.8	4.4	0.0128588
30	8.955	730	94.1	0.0160998	8	0.211896	0.9	0.8	8.955	733	7.9	0.0150929

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	20	2.6	0.0326334	6	0.344251	5.4	4.2	4.55	21.2	5.7	0.0338736
2	5.2	111	14.3	0.0457419	8	0.667874	10.6	8.1	5.2	109.8	8.2	0.0461272
3	5.85	221	28.4	0.0835782	5	0.760301	12	9.2	5.85	221.3	5	0.0861005
4	6.55	371	47.7	0.0914026	8	1.20071	19	14.6	6.55	369.1	8	0.0851067
5	6.85	407	52.3	0.02385	5	0.202364	3.2	2.5	6.85	407.2	5.4	0.0211987
6	7.35	472	60.7	0.0504332	4	0.368478	5.8	4.5	7.35	471.3	4.3	0.048054
7	8.15	593	76.2	0.0631464	8	0.851641	13.5	10.3	8.15	593	7.9	0.060615
8	8.45	663	85.2	0.0852853	7	1.00177	15.9	12.2	8.45	662.6	6.8	0.0832091
9	8.65	711	91.4	0.0807116	4	0.558782	8.8	6.8	8.65	710.4	4.1	0.077706
10	9.3	754	96.9	0.0381983	6	0.361019	5.7	4.4	9.3	752.7	6.1	0.0331833

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.4	0.0237161	5	0.199225	1	1	4.55	19.2	5	0.02257
2	4.724	45	5.8	0.00989299	4	0.0730114	0.4	0.3	4.724	45.8	4.1	0.00998469
3	5.2	116	14.9	0.0328565	6	0.348196	1.8	1.7	5.2	115.1	6	0.0328776
4	5.544	170	21.9	0.00831188	3	0.0411016	0.2	0.2	5.544	170.2	2.7	0.00868886
5	5.774	206	26.5	0.0322451	3	0.192206	1	0.9	5.774	206.3	3.4	0.0321363
6	5.85	218	28	0.061481	4	0.409342	2.1	2	5.85	218.4	3.7	0.0624368
7	5.995	249	32	0.00907506	3	0.0405373	0.2	0.2	5.995	249.1	3	0.00751853
8	6.084	268	34.4	0.0148523	5	0.11575	0.6	0.6	6.084	268.3	4.8	0.0136803
9	6.192	291	37.4	0.140732	4	1.03772	5.3	5	6.192	291	4	0.146407
10	6.277	309	39.7	0.0586064	3	0.355444	1.8	1.7	6.277	308.8	3.3	0.0598647
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.361	327	42	0.0593756	4	0.445341	2.3	2.1	6.361	326.1	4.4	0.0566154
12	6.422	340	43.7	0.0494369	3	0.231031	1.2	1.1	6.422	340.1	2.9	0.0447777
13	6.53	363	46.7	0.0477037	4	0.333366	1.7	1.6	6.53	361.8	4.3	0.0433785
14	6.589	372	47.8	0.288704	2	0.899725	4.6	4.3	6.589	371.9	1.8	0.283701
15	6.64	378	48.6	0.0615719	2	0.17952	0.9	0.9	6.64	377.8	1.6	0.0635552
16	6.682	383	49.2	0.286726	2	1.07699	5.6	5.1	6.682	383.5	1.9	0.323092
17	6.741	390	50.1	0.0554799	1	0.127599	0.7	0.6	6.741	390.1	1.3	0.0548581
18	6.791	396	50.9	0.443154	2	1.80298	9.3	8.6	6.791	396.6	2.2	0.461275
19	6.931	414	53.2	0.179534	3	0.900774	4.6	4.3	6.931	413.9	2.9	0.17319
20	7.019	426	54.8	0.109519	5	0.347914	1.8	1.7	7.019	422.9	5	0.0393111
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.085	435	55.9	0.126926	10	2.16139	11.1	10.3	7.085	433.2	10.2	0.119074
22	7.225	454	58.4	0.377059	2	1.49331	7.7	7.1	7.225	454.4	2.2	0.379945
23	7.35	471	60.5	0.0684455	4	0.424184	2.2	2	7.35	470.2	3.9	0.061562
24	7.547	501	64.4	0.0721309	18	2.09366	10.8	10	7.547	500.2	18	0.0656247
25	7.822	543	69.8	0.0651877	3	0.286228	1.5	1.4	7.822	542.6	2.5	0.0639758
26	7.92	558	71.7	0.37457	3	1.85937	9.6	8.9	7.92	557.7	2.7	0.382808
27	8.15	593	76.2	0.0333726	8	0.473172	2.4	2.3	8.15	594	8.4	0.0318084
28	8.324	636	81.7	0.01032	5	0.075217	0.4	0.4	8.324	635	5.1	0.00837009
29	8.377	649	83.4	0.0178598	3	0.105781	0.5	0.5	8.377	648.1	3.4	0.0176182
30	8.45	667	85.7	0.0433331	8	0.571547	2.9	2.7	8.45	665.8	7.6	0.0423386
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.534	686	88.2	0.0168499	6	0.155884	0.8	0.7	8.534	685.7	5.7	0.0153776
32	8.65	712	91.5	0.0574612	6	0.540905	2.8	2.6	8.65	711.4	5.7	0.0534398

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.78	50	6.4	0.0136635	5	0.115619	0.4	0.4	4.78	49.5	4.9	0.0132245
2	5.589	176	22.6	0.0186307	4	0.113176	0.4	0.4	5.589	175.9	3.7	0.0170896
3	5.805	212	27.2	0.0691666	3	0.392437	1.5	1.3	5.805	212.3	3.1	0.0709839
4	6.007	252	32.3	0.00805027	4	0.0428204	0.2	0.1	6.007	252.4	3.7	0.00659791
5	6.098	271	34.8	0.0452859	4	0.318014	1.2	1.1	6.098	270.5	3.9	0.0461323
6	6.204	293	37.6	0.267534	4	1.71606	6.5	5.7	6.204	292.8	3.6	0.271031
7	6.282	309	39.7	0.096619	3	0.531891	2	1.8	6.282	309	3	0.0993198
8	6.359	325	41.7	0.0901323	5	0.774356	3	2.6	6.359	324.2	4.7	0.0926522
9	6.417	337	43.3	0.0803288	4	0.517011	2	1.7	6.417	336.4	3.5	0.0832272
10	6.508	356	45.7	0.0640756	5	0.474253	1.8	1.6	6.508	355.7	4.6	0.0575615
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.587	369	47.4	0.207071	3	1.177	4.5	3.9	6.587	368.2	3.1	0.214589
12	6.638	375	48.1	0.0682034	4	0.687889	2.6	2.3	6.638	377.5	4.3	0.0893025
13	6.689	381	48.9	0.239522	2	0.784886	3	2.6	6.689	381.1	2.2	0.200212
14	6.748	388	49.8	0.0972783	2	0.413046	1.6	1.4	6.748	387.9	2.3	0.100427
15	6.808	395	50.7	0.419539	3	2.11916	8.1	7	6.808	395.2	2.8	0.422794
16	6.944	413	53	0.216521	3	1.04535	4	3.5	6.944	413.2	2.9	0.202224
17	7.031	425	54.6	0.113366	8	1.45369	5.5	4.8	7.031	423.9	7.7	0.107178
18	7.111	436	56	0.224274	6	2.21079	8.4	7.3	7.111	436.6	6	0.207204
19	7.234	453	58.2	0.418471	2	1.76176	6.7	5.9	7.234	453	2.3	0.42551
20	7.343	468	60.1	0.0241824	4	0.167374	0.6	0.6	7.343	468.2	4.1	0.023112
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.569	503	64.6	0.0776835	18	3.59684	13.7	11.9	7.569	532.9	18	0.112741
22	7.834	544	69.8	0.108379	1	0.0277917	0.1	0.1	7.834	543.4	0.8	0.0196495
23	7.924	558	71.6	0.459089	3	2.25281	8.6	7.5	7.924	558.4	2.9	0.430963
24	8.361	643	82.5	0.049284	12	0.952552	3.6	3.2	8.361	643.5	11.7	0.0460248
25	8.412	655	84.1	0.0794308	5	0.515602	2	1.7	8.412	655.7	5	0.0585681
26	8.454	665	85.4	0.0731514	6	0.811942	3.1	2.7	8.454	666.4	6.5	0.0707387
27	8.518	680	87.3	0.0596092	7	0.669582	2.6	2.2	8.518	682.2	7	0.0543231
28	8.573	693	89	0.0268359	2	0.046181	0.2	0.2	8.573	693.3	2.3	0.0114347
29	8.71	715	91.8	0.0129478	5	0.107105	0.4	0.4	8.71	716.2	4.6	0.0132673
30	9.04	737	94.6	0.0299605	7	0.403746	1.5	1.3	9.04	736.9	7.2	0.0315527

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	13	1.7	0.0738797	4	0.443824	5.5	4.1	4.55	13.4	3.5	0.0710917
2	5.2	105	13.5	0.067862	5	0.580292	7.2	5.3	5.2	105.2	5.2	0.0629333
3	5.85	220	28.2	0.121551	4	0.785671	9.8	7.2	5.85	220.1	3.7	0.120492
4	6.55	361	46.3	0.107704	8	1.51203	18.8	13.9	6.55	360.1	8.3	0.102779
5	6.85	396	50.8	0.0272964	5	0.211923	2.6	1.9	6.85	396.4	4.7	0.0256595
6	7.35	466	59.8	0.0753406	4	0.524678	6.5	4.8	7.35	465.4	4.1	0.0716215
7	8.15	592	76	0.111562	7	1.35678	16.9	12.4	8.15	592	7.1	0.108455
8	8.45	660	84.7	0.119129	7	1.4262	17.7	13.1	8.45	659.9	7	0.114752
9	8.65	709	91	0.105879	5	0.853884	10.6	7.8	8.65	708.5	4.8	0.101184
10	9.3	754	96.8	0.0317974	8	0.349308	4.3	3.2	9.3	751.1	8.2	0.0239123

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 122

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.900 5.200 8.800 5.850 11.500 6.550 18.800 6.850 4.300 7.350 6.100 8.150 13.100 8.450 16.300 8.650 10.000 9.300 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.802 0.500 5.577 1.700 5.784 1.700 6.071 1.200 6.181 6.900 6.271 2.400 6.357 3.000 6.419 1.600 6.510 1.500 6.524 1.200 6.588 4.300 6.638 2.800 6.679 4.200 6.729 0.900 6.796 8.400 6.930 4.400 7.029 5.100 7.106 8.200 7.235 7.400 7.357 0.600 7.558 13.500 7.805 1.400 7.903 10.800 8.379 0.600 8.435 1.200 8.481 1.100 8.535 1.200 8.573 0.800 8.736 0.400 8.955 0.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 30 Return to Lane Navigator
4.550 5.400 5.200 10.600 5.850 12.000 6.550 19.000 6.850 3.200 7.350 5.800 8.150 13.500 8.450 15.900 8.650 8.800 9.300 5.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 1.000 4.724 0.400 5.200 1.800 5.544 0.200 5.774 1.000 5.850 2.100 5.995 0.200 6.084 0.600 6.192 5.300 6.277 1.800 6.361 2.300 6.422 1.200 6.530 1.700 6.589 4.600 6.640 0.900 6.682 5.600 6.741 0.700 6.791 9.300 6.931 4.600 7.019 1.800 7.085 11.100 7.225 7.700 7.350 2.200 7.547 10.800 7.822 1.500 7.920 9.600 8.150 2.400 8.324 0.400 8.377 0.500 8.450 2.900 8.534 0.800 8.650 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 32 Return to Lane Navigator
4.780 0.400 5.589 0.400 5.805 1.500 6.007 0.200 6.098 1.200 6.204 6.500 6.282 2.000 6.359 3.000 6.417 2.000 6.508 1.800 6.587 4.500 6.638 2.600 6.689 3.000 6.748 1.600 6.808 8.100 6.944 4.000 7.031 5.500 7.111 8.400 7.234 6.700 7.343 0.600 7.569 13.700 7.834 0.100 7.924 8.600 8.361 3.600 8.412 2.000 8.454 3.100 8.518 2.600 8.573 0.200 8.710 0.400 9.040 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 30 Return to Lane Navigator
4.550 5.500 5.200 7.200 5.850 9.800 6.550 18.800 6.850 2.600 7.350 6.500 8.150 16.900 8.450 17.700 8.650 10.600 9.300 4.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Escolar(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Escolar(*) ---- Go to Display Gel Lane Navigator.