RFE Fish Species Identification Isoelectric Focusing Tools - Common Thresher Shark Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Common Thresher Shark ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Alopias vulpinis
FDA Seafood List Common Name: Common Thresher Shark
FDA Seafood List Market Name: Shak, Thresher
Unique IEF Gel Code: 19970219gel04
Unique Web Code: cth01Srr
Tissue Aliquot Code: alopias I94-001 #04 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.0793336	4	0.493894	5.5	4.2	4.55	24.4	3.7	0.0746485
2	5.2	112	14.5	0.114667	4	0.797521	8.9	6.8	5.2	112	4.2	0.107195
3	5.85	213	27.7	0.103873	4	0.702521	7.9	6	5.85	212.9	3.8	0.104952
4	6.55	348	45.2	0.125444	6	1.31553	14.7	11.2	6.55	347.7	6.2	0.119558
5	6.85	379	49.2	0.0459675	4	0.320746	3.6	2.7	6.85	379	4.4	0.0413207
6	7.35	441	57.3	0.0956387	4	0.618611	6.9	5.3	7.35	440.6	3.6	0.095682
7	8.15	566	73.5	0.129999	7	1.51151	16.9	12.8	8.15	565.3	6.7	0.127577
8	8.45	639	83	0.158756	6	1.62508	18.2	13.8	8.45	638.3	5.9	0.15491
9	8.65	699	90.8	0.11338	4	0.8048	9	6.8	8.65	699.1	4.2	0.108762
10	9.3	749	97.3	0.0932082	4	0.743411	8.3	6.3	9.3	749.1	4.5	0.0942471

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.568	25	3.3	0.0112239	4	0.0788829	0.4	0.4	4.568	24.5	3.9	0.0113286
2	5.276	125	16.3	0.00411949	3	0.0347394	0.2	0.2	5.276	126.7	3.3	0.00591209
3	5.656	183	23.8	0.00767	4	0.0417845	0.2	0.2	5.656	183.1	3.5	0.00670246
4	6.01	244	31.7	0.0170913	5	0.13312	0.7	0.7	6.01	243.9	5.2	0.0144014
5	6.173	276	35.9	0.0121353	5	0.0933997	0.5	0.5	6.173	277.6	4.6	0.0114178
6	6.229	287	37.3	0.00950114	3	0.044819	0.2	0.2	6.229	287.1	2.7	0.00922051
7	6.295	300	39	0.0107249	5	0.094357	0.5	0.5	6.295	300.3	5.1	0.0103978
8	6.372	315	41	0.0194985	5	0.136042	0.7	0.7	6.372	316.7	4.6	0.0168454
9	6.423	325	42.3	0.0107627	2	0.0409807	0.2	0.2	6.423	325.5	2.4	0.00983716
10	6.494	339	44.1	0.00697783	3	0.0346642	0.2	0.2	6.494	339.3	2.7	0.00725012
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.535	347	45.1	0.018433	3	0.0873966	0.5	0.4	6.535	346.7	2.7	0.0185464
12	6.724	368	47.9	0.142222	4	1.00172	5.3	5.1	6.724	367.9	4.2	0.135378
13	6.85	381	49.5	0.485944	3	2.61969	14	13.3	6.85	380.8	3	0.48647
14	6.939	392	51	0.177849	5	1.53174	8.2	7.8	6.939	391.4	5	0.17292
15	7.118	414	53.8	0.0246976	3	0.139249	0.7	0.7	7.118	414.1	3	0.0261226
16	7.224	427	55.5	0.0662784	3	0.369352	2	1.9	7.224	426.9	3.1	0.0678921
17	7.322	439	57.1	0.0393905	8	0.544171	2.9	2.8	7.322	443.5	7.6	0.0404192
18	7.406	451	58.6	0.0773133	3	0.303194	1.6	1.5	7.406	451.1	2.8	0.0613183
19	7.471	461	59.9	0.0295222	4	0.138522	0.7	0.7	7.471	462.3	3.9	0.0202449
20	7.621	484	62.9	0.130559	12	2.42846	13	12.3	7.621	483.9	12.3	0.111323
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.876	523	68	0.0131787	3	0.0884578	0.5	0.4	7.876	524.3	3.5	0.0143637
22	7.948	534	69.4	0.0621444	4	0.396647	2.1	2	7.948	534.1	3.8	0.0586516
23	8.17	570	74.1	0.04667	9	0.684209	3.7	3.5	8.17	571.9	8.5	0.0453046
24	8.263	593	77.1	0.0489211	10	0.808288	4.3	4.1	8.263	591.3	9.7	0.0469176
25	8.323	608	79.1	0.00785574	1	0	0	0	8.323	604	1	0
26	8.396	626	81.4	0.28427	4	2.00198	10.7	10.2	8.396	625.7	4.1	0.276586
27	8.51	657	85.4	0.0321013	6	0.303925	1.6	1.5	8.51	657.6	5.6	0.0307285
28	8.568	674	87.6	0.00879012	5	0.0918393	0.5	0.5	8.568	676.2	5.1	0.0100677
29	8.883	715	93	0.424171	6	4.46752	23.8	22.7	8.883	714.2	5.6	0.446976

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	25	3.2	0.0546394	5	0.478789	4.4	3.5	4.55	24.7	5.1	0.0524881
2	5.2	119	15.4	0.0735207	7	0.784748	7.3	5.7	5.2	118.1	6.7	0.0665186
3	5.85	216	28	0.122918	4	0.924658	8.5	6.7	5.85	216.1	4.2	0.124963
4	6.55	356	46.1	0.175691	7	1.99656	18.5	14.4	6.55	355.4	6.7	0.167878
5	6.85	387	50.1	0.0668199	4	0.482038	4.5	3.5	6.85	387.5	4.3	0.0639592
6	7.35	448	58	0.144252	4	0.980681	9.1	7.1	7.35	447.9	3.9	0.142672
7	8.15	568	73.6	0.125497	8	1.66632	15.4	12	8.15	567.8	7.7	0.122506
8	8.45	644	83.4	0.149658	6	1.66106	15.4	12	8.45	643.5	6.3	0.148255
9	8.65	701	90.8	0.11709	5	0.910321	8.4	6.6	8.65	700.8	4.5	0.113648
10	9.3	746	96.6	0.132951	4	0.933228	8.6	6.7	9.3	745.7	4	0.131769

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	23	3	0.0469902	5	0.396879	1.9	1.8	4.55	21.9	4.8	0.0462376
2	5.2	111	14.5	0.0535935	7	0.628903	3	2.9	5.2	110.3	6.9	0.0517367
3	5.635	178	23.2	0.00474952	5	0.0503406	0.2	0.2	5.635	178.2	4.9	0.00580269
4	5.85	211	27.5	0.0642357	5	0.541501	2.5	2.5	5.85	210.1	4.8	0.0631723
5	6.004	241	31.4	0.0148988	6	0.129904	0.6	0.6	6.004	240.7	6	0.0122377
6	6.169	273	35.5	0.00787361	4	0.0578573	0.3	0.3	6.169	274.1	4.3	0.00751087
7	6.221	283	36.8	0.006192	4	0.0391298	0.2	0.2	6.221	282.8	3.6	0.00606496
8	6.303	299	38.9	0.00609148	5	0.04845	0.2	0.2	6.303	297.6	5.2	0.00529949
9	6.37	312	40.6	0.0154393	4	0.0987363	0.5	0.5	6.37	313.9	4.2	0.013279
10	6.421	322	41.9	0.00615905	1	0.0189664	0.1	0.1	6.421	323.1	1.1	0.00963511
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.457	329	42.8	0.00645711	4	0.0332442	0.2	0.2	6.457	329.6	4	0.00464849
12	6.55	347	45.2	0.0600045	5	0.507566	2.4	2.3	6.55	346.5	4.7	0.0611408
13	6.738	367	47.8	0.14335	4	0.888993	4.2	4.1	6.738	366.8	3.8	0.130901
14	6.858	380	49.5	0.512104	3	2.61817	12.3	12.1	6.858	380.4	2.9	0.506298
15	6.959	393	51.2	0.172657	5	1.37987	6.5	6.4	6.959	391.8	4.9	0.159684
16	7.053	405	52.7	0.0106959	2	0.0253211	0.1	0.1	7.053	406	1.9	0.00759811
17	7.147	417	54.3	0.0313314	3	0.173361	0.8	0.8	7.147	417.1	3.2	0.0304685
18	7.256	431	56.1	0.0575083	3	0.290091	1.4	1.3	7.256	430.3	2.7	0.0595807
19	7.35	443	57.7	0.114205	3	0.64158	3	3	7.35	442.9	3.3	0.1088
20	7.43	455	59.2	0.055395	6	0.446191	2.1	2.1	7.43	453.5	5.6	0.0450052
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.497	465	60.5	0.0298355	6	0.210713	1	1	7.497	469.3	6	0.0197469
22	7.643	487	63.4	0.115044	12	2.15545	10.1	9.9	7.643	486.9	11.6	0.10524
23	7.903	526	68.5	0.00600545	2	0.0151375	0.1	0.1	7.903	525.8	1.8	0.0048307
24	7.977	537	69.9	0.0313845	4	0.197174	0.9	0.9	7.977	537.4	3.6	0.0308589
25	8.15	563	73.3	0.0322916	5	0.1405	0.7	0.6	8.15	559.3	4.5	0.0175169
26	8.182	571	74.3	0.0348338	11	0.618335	2.9	2.8	8.182	570.3	11.3	0.0309638
27	8.278	595	77.5	0.0129616	3	0.0572363	0.3	0.3	8.278	595.7	3.1	0.0104627
28	8.334	609	79.3	0.0163532	6	0.159117	0.7	0.7	8.334	611	5.8	0.0154809
29	8.398	625	81.4	0.214196	5	1.82636	8.6	8.4	8.398	625.2	4.8	0.214833
30	8.45	638	83.1	0.0458314	7	0.536049	2.5	2.5	8.45	639.1	6.6	0.0456097
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.555	669	87.1	0.0252408	8	0.288628	1.4	1.3	8.555	667.9	7.8	0.0207957
32	8.65	697	90.8	0.0739424	15	1.68653	7.9	7.8	8.65	698.8	15.1	0.0631348
33	8.94	718	93.5	0.431406	5	3.91745	18.4	18	8.94	717.4	5.3	0.419408
34	9.3	744	96.9	0.0629403	4	0.429717	2	2	9.3	743.3	3.9	0.0616391

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.576	24	3.1	0.00791195	9	0.0880448	0.4	0.4	4.576	24	8.6	0.00579739
2	5.275	119	15.5	0.00545968	6	0.0457044	0.2	0.2	5.275	118.9	6.1	0.00423719
3	5.65	179	23.3	0.00672741	4	0.0364071	0.2	0.2	5.65	178.9	3.6	0.00576773
4	6.001	240	31.3	0.0122668	6	0.123477	0.5	0.5	6.001	241.1	5.6	0.0125294
5	6.173	273	35.6	0.0119919	4	0.0786967	0.3	0.3	6.173	273.3	4.3	0.0103331
6	6.287	295	38.5	0.00667633	5	0.0532064	0.2	0.2	6.287	296.9	4.9	0.00616966
7	6.37	311	40.5	0.0162711	3	0.092651	0.4	0.4	6.37	311.2	3.2	0.016137
8	6.391	315	41.1	0.0147883	1	0.0237375	0.1	0.1	6.391	315.2	1.3	0.0104959
9	6.428	322	42	0.00357395	1	0	0	0	6.428	318	1	0
10	6.485	333	43.4	0.00920196	3	0.048307	0.2	0.2	6.485	333.3	3.1	0.00881602
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.527	341	44.5	0.0207657	3	0.0957822	0.4	0.4	6.527	340.7	2.6	0.0208409
12	6.708	361	47.1	0.153857	4	1.12367	5	4.7	6.708	360.5	4.4	0.144848
13	6.83	373	48.6	0.403401	3	2.40882	10.7	10.2	6.83	373.1	3.4	0.400505
14	6.934	386	50.3	0.13149	5	1.08082	4.8	4.6	6.934	384.9	4.8	0.125985
15	7.018	397	51.8	0.00963894	2	0.0166654	0.1	0.1	7.018	398.3	1.7	0.00563474
16	7.125	411	53.6	0.0232883	4	0.159892	0.7	0.7	7.125	411.2	3.8	0.0239376
17	7.239	426	55.5	0.0609693	3	0.380111	1.7	1.6	7.239	425.4	3.4	0.0623451
18	7.346	440	57.4	0.0448394	5	0.298108	1.3	1.3	7.346	439.4	4.5	0.0371079
19	7.424	452	58.9	0.101182	6	1.07611	4.8	4.5	7.424	450.2	6.4	0.094826
20	7.495	463	60.4	0.0682983	8	0.869879	3.9	3.7	7.495	465	7.8	0.0626625
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.636	485	63.2	0.150294	11	2.63845	11.7	11.1	7.636	483.7	10.9	0.136262
22	7.739	501	65.3	0.0612877	8	0.73295	3.2	3.1	7.739	498	7.7	0.053515
23	7.899	526	68.6	0.0129177	3	0.0569795	0.3	0.2	7.899	524.5	2.7	0.0120836
24	7.97	537	70	0.0563121	7	0.591088	2.6	2.5	7.97	537.8	6.7	0.05009
25	8.203	578	75.4	0.0484383	7	0.491941	2.2	2.1	8.203	577.6	6.9	0.0399595
26	8.261	592	77.2	0.107398	8	1.51859	6.7	6.4	8.261	590.9	8.2	0.104556
27	8.31	604	78.7	0.0594039	7	0.573513	2.5	2.4	8.31	604.5	6.6	0.0493665
28	8.384	622	81.1	0.289011	6	2.97825	13.2	12.6	8.384	622.9	6.2	0.272776
29	8.521	659	85.9	0.0335351	9	0.469403	2.1	2	8.521	657.6	8.9	0.0297976
30	8.582	677	88.3	0.0105996	2	0.0335524	0.1	0.1	8.582	677.3	2.4	0.00786324
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.619	688	89.7	0.0142464	3	0.0698983	0.3	0.3	8.619	688.3	2.7	0.0143703
32	8.905	716	93.4	0.454184	5	4.31094	19.1	18.2	8.905	715.5	5.3	0.456867

PeakCounter: 32

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	17	2.2	0.0736471	5	0.577748	5.2	4	4.55	17.2	4.5	0.0718659
2	5.2	102	13.3	0.08783	7	1.03954	9.4	7.2	5.2	102.4	6.7	0.0879901
3	5.85	210	27.5	0.129637	4	0.888193	8.1	6.2	5.85	210.3	3.8	0.131858
4	6.55	343	44.8	0.186216	6	1.93433	17.6	13.5	6.55	342.3	6.2	0.176246
5	6.85	370	48.4	0.0912699	5	0.709386	6.4	4.9	6.85	370.4	4.8	0.0840337
6	7.35	437	57.1	0.142931	4	0.969737	8.8	6.8	7.35	437.2	3.8	0.142121
7	8.15	566	74	0.122963	7	1.46119	13.3	10.2	8.15	565.6	6.8	0.121044
8	8.45	637	83.3	0.136334	7	1.59772	14.5	11.1	8.45	636.4	6.6	0.136149
9	8.65	696	91	0.120858	5	0.961788	8.7	6.7	8.65	696.4	4.6	0.117409
10	9.3	746	97.5	0.116603	4	0.867174	7.9	6	9.3	746.1	4.1	0.118236

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 125

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.500 5.200 8.900 5.850 7.900 6.550 14.700 6.850 3.600 7.350 6.900 8.150 16.900 8.450 18.200 8.650 9.000 9.300 8.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.568 0.400 5.276 0.200 5.656 0.200 6.010 0.700 6.173 0.500 6.229 0.200 6.295 0.500 6.372 0.700 6.423 0.200 6.494 0.200 6.535 0.500 6.724 5.300 6.850 14.000 6.939 8.200 7.118 0.700 7.224 2.000 7.322 2.900 7.406 1.600 7.471 0.700 7.621 13.000 7.876 0.500 7.948 2.100 8.170 3.700 8.263 4.300 8.323 0 8.396 10.700 8.510 1.600 8.568 0.500 8.883 23.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 29 Return to Lane Navigator
4.550 4.400 5.200 7.300 5.850 8.500 6.550 18.500 6.850 4.500 7.350 9.100 8.150 15.400 8.450 15.400 8.650 8.400 9.300 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 1.900 5.200 3.000 5.635 0.200 5.850 2.500 6.004 0.600 6.169 0.300 6.221 0.200 6.303 0.200 6.370 0.500 6.421 0.100 6.457 0.200 6.550 2.400 6.738 4.200 6.858 12.300 6.959 6.500 7.053 0.100 7.147 0.800 7.256 1.400 7.350 3.000 7.430 2.100 7.497 1.000 7.643 10.100 7.903 0.100 7.977 0.900 8.150 0.700 8.182 2.900 8.278 0.300 8.334 0.700 8.398 8.600 8.450 2.500 8.555 1.400 8.650 7.900 8.940 18.400 9.300 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 34 Return to Lane Navigator
4.576 0.400 5.275 0.200 5.650 0.200 6.001 0.500 6.173 0.300 6.287 0.200 6.370 0.400 6.391 0.100 6.428 0 6.485 0.200 6.527 0.400 6.708 5.000 6.830 10.700 6.934 4.800 7.018 0.100 7.125 0.700 7.239 1.700 7.346 1.300 7.424 4.800 7.495 3.900 7.636 11.700 7.739 3.200 7.899 0.300 7.970 2.600 8.203 2.200 8.261 6.700 8.310 2.500 8.384 13.200 8.521 2.100 8.582 0.100 8.619 0.300 8.905 19.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 32 Return to Lane Navigator
4.550 5.200 5.200 9.400 5.850 8.100 6.550 17.600 6.850 6.400 7.350 8.800 8.150 13.300 8.450 14.500 8.650 8.700 9.300 7.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Common Thresher Shark ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Common Thresher Shark ---- Go to Display Gel Lane Navigator.