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Research Laboratories
Flow Cytometry Core Laboratory
Mario Roederer, Ph.D.
Quantum dots suspended in PBS
and placed into the 407nm Violet DPSS laser. The emission
shows the wide range of the visible spectrum, which
can be measured from this single laser |
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Personnel:
Mario Roederer, Director
telephone: (301)-594-8491; fax(301)
480-2651; e-mail: Roederer@nih.gov
Stephen Perfetto, Manager
telephone: (301)-594-8659; fax: (301) 480-2651; e-mail:
Sperfetto@nih.gov
Richard Nguyen, Specialist
e-mail: rnguyen@nih.gov
David Ambrozak, Specialist
e-mail: dambrozak@nih.gov
Address: VRC, NIH, 40 Convent Dr.,
Room 5508, Bethesda, MD, 20892-3015
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From Left to Right: Stephen
Perfetto, Mario Roederer,
Richard Nguyen, and David Ambrozak. |
Description The VRC Flow Cytometry
Core Facility contains seven instruments with capabilities of
serving a wide range of flow cytometric clinical and research
demands. Currently, the laboratory has three FACSCaliburs, which can
measure up to 4-colors and these are equipped with a HTS (High
Throughput Sampler) device that aids in fast acquisition of
specimens from 96 well tray. The FACSDiVa sorter is capable of
high-speed, high-end multicolor analysis, measuring 18 parameters.
Two LSR II instruments are equipped with HTS units and are capable
of measuring 20 parameters. Additionally, the FACSAria is a 20
parameter high-speed sorter located in our BSL-3 facility and is
capable of infectious cell sorting. (Link to Instrumentation for
more detail).
The VRC Flow Cytometry Core Facility services
the research requirements of the VRC investigators as well as the
clinical needs of the vaccine trials supported by the VRC. In
addition, we offer limited collaborations to NIH investigators and
other outside facilities as determined by the director. Please
contact Stephen Perfetto or Mario Roederer for more information.
VRC Flow Cytometry Core Facility Usage and
Policies Usage within the VRC: All VCR users access
electronic sign-up calendars located in the public folder on the
Outlook Mail Server for all immunophenotyping procedures. Sorting
procedures are scheduled through the VRC core facility personnel
only.
Collaboration outside of the VRC: Access to the
facility is limited to those who collaborate with VRC investigators.
Please contact Mario Roederer for more information.
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