Harlan Animal Colonies Health Evaluation Summary: 12/19/06
Contract N01-AG-7-2113
RATS Barrier 202B
Animal Facility | Strain | No.Sex | Age | Testing Laboratory/Dates |
---|
Location: Indianapolis, IN | F344/NHsd | 1M/0F | 5 months | University of Missouri |
Barrier/Building: 202B | | 1M/0F | 12 months | MURADIL, Columbia, MO |
| | 0M/1F | 18 months | |
| | | | Receipt Date: 10/23/06 |
Ship Date: 10/22/06 | | | | Report Date: 12/13/06 |
Evaluation
NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan. All rats examined were considered to be in a good state of health. |
Serology
Agent | Assay | No. Pos./No. Tested | Comments |
---|
Sendai | MFI | 0/3 | |
RCV/SDAV | MFI | 0/3 | |
Parvoviruses | MFI | 0/3 | |
KRV | MFI | 0/3 | |
H-1 | MFI | 0/3 | |
PVM | MFI | 0/3 | |
M. pulmonis | MFI | 0/3 | |
Reo 3 | MFI | 0/0* | |
TMEV GDVII | MFI | 0/0* | |
MAD | MFI | 0/0* | |
LCM | MFI | 0/0* | |
Hantaan | MFI | 0/0* | *Previously Tested Negative |
Bacteriology
Agent | No. Pos./No. Tested | Comments |
---|
Mycoplasma pulmonis | 0/3 | |
Salmonella spp. | 0/3 | Nasopharynx/lung/cecum/uterine cultures |
Pasturella pneumotropica | 0/3 | |
Pasturella spp. | 0/3 | |
Bordetella bronchiseptica | 0/3 | |
Pseudomonas aeruginosa | 0/3 | |
Citrobacter rodentium | 0/3 | |
Corynebaterium kutscheri | 0/3 | |
Klebsiella pneumoniae | 2/3 | |
Klebsiella oxytoca | 0/3 | The bacteria isolated from the nasopharynx and cecum are opportunistic or normal flora of the rat. |
Staphylococcus aureus | 0/3 |
Staphylococcus pneumoniae | 0/3 | |
Streptococcus sp. Group B beta | 0/3 | |
PCR
| No. Pos./No.Tested | Comments |
---|
Mycoplasma pulmonis | 0/3 | |
Heliobacter hepaticus | 0/3 | |
Parasitology
Type and Test | Results | Comments |
---|
Ectoparasites | No. Pos./No. Tested | External parasites were not observed in any rat. |
Test: Direct pelage
| 0/3 |
Endoparasites | No. Pos./No. Tested | Enteric and urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestines & bladder)
| 0/3 |
Pathology
Gross: | None Significant | Overall frequency of lesions in these rats is low. |
---|
Histologic: | | |
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Lymphoid infiltrate lung | 3/3 | Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
Harderian gland lymphoid infiltrate | 2/3 |
Microgranuloma liver | 3/3 |
Focal myocardial degeneration | 3/3 |
A few other incidental lesions were also noted. | | |
Availability
Rats obtained from NIA-NIH aging colonies maintained in a maximum security barrier by Harlan in Indianapolis, Indiana. The combined census of these colonies, at the time of the referenced monitoring, is tabulated below: |
Retired Breeder - Male (9-26 months of age) | 170 | |
Virgin - Male (1-28 months of age) | 3,616 | |
Virgin - Female (1-32 months of age)
| 2,133
| |
Total Census:
| 5,919 | |
For information, contact:
Dr. Karla A. Stevens
HARLAN SPRAGUE DAWLEY, INC.
8520 Allison Pointe, #400
Indianapolis, IN 46250
Phone: (317) 806-6060
Fax: (317) 806-6073
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Jun 20, 2008