King County Navigation Bar (text navigation at bottom)
Public Health - Seattle & King County
Site Directory

Public Health Webpage Directory

Public Health Center & Office Locations

For Care Providers

Health Advisories & Resources

For Educators

Health Educators Toolbox

About Us

History & Profile

Jobs

Employee Directory

Contact Us

Public Health
Seattle & King County
401 5th Ave., Suite 1300
Seattle, WA 98104

Click here to email us

Phone: 206-296-4600
TTY Relay: 711

magnifying glass Advanced Search
Search Tips
Home » Health Care Providers homepage » West Nile virus

Health Advisories & Resources
West Nile virus facts for Health Care Providers

News and alerts

Transmission

  • West Nile virus (WNV) is a flavivirus related to the Japanese encephalitis and St. Louis encephalitis viruses.
  • WNV can infect humans, horses, birds, and other vertebrates.
  • WNV is transmitted by the bite of an infected mosquito.
  • WNV is not transmitted by person-to-person contact.
  • WNV has been transmitted to humans through blood transfusions and organ transplantation; single cases of transplacental and breast milk transmission have also been reported.

Clinical presentation

  • Incubation time is between 2 and 14 days.
  • Most people with WNV have mild symptoms or are asymptomatic.
  • Approximately 20% of infected persons develop a mild form of the disease, called West Nile fever. Symptoms of West Nile fever may include fever, malaise, anorexia, nausea, vomiting, myalgia, eye pain, rash, and swollen lymph glands.
  • One in 150 infections results in the more severe neuroinvasive form of the disease, including meningitis and encephalitis. Symptoms of neuroinvasive disease may include: fever, headache, neck stiffness, gastrointestinal symptoms, disorientation, cranial nerve abnormalities, ataxia, coma, tremors, convulsions, muscle weakness, and paralysis.
  • Approximately 3-15% of patients hospitalized with West Nile neuroinvasive disease die from the disease. The risk of mortality is highest in elderly patients.
  • Consider WNV in the differential diagnosis of all patients with meningitis and/or encephalitis of unknown etiology during mosquito season, particularly in elderly patients presenting with weakness or acute flaccid paralysis or presumed Guillain-Barré syndrome.

Diagnosis and laboratory testing

Diagnosis

  • The best way to diagnose WNV is to test for IgM antibody to WNV in serum collected between 8 and 14 days of illness onset or cerebrospinal fluid (CSF) collected within 8 days of onset using the IgM antibody-capture, enzyme immunoassay (EIA). Because IgM does not cross the blood brain barrier, its presence in CSF indicates neuroinvasive disease.
  • IgM antibody can persist for many months and may be indicative of a past infection.
  • The West Nile virus IgM antibody-capture EIA test can exhibit cross-reactivity in patients who have recently been vaccinated against or recently infected with related flaviviruses (e.g. yellow fever, Japanese encephalitis, dengue).
  • It is difficult to clinically distinguish WNV from other causes of meningoencephalitis, so testing for other potential causes of aseptic meningitis (enteroviruses and herpes viruses) is suggested. Also consider additional testing if a patient’s travel history is suggestive of other arboviral exposure.

Criteria for testing WNV specimens at the Washington State Department of Health Public Health Laboratory (WA DOH PHL). NOTE: Public Health-Seattle & King County will facilitate testing at the WA DOH PHL.

  • Patients with suspected WNV neuroinvasive disease (fever and change in mental status, CSF pleocytosis, or other acute central or peripheral neurologic dysfunction) when there is no other likely diagnosis.
  • Pregnant or breastfeeding women symptomatic with suspected WNV infection and their neonates or breastfeeding infants.
  • Recent blood, tissue, or organ donors or recipients suspected to have WNV infection.
  • Persons with commercial laboratory evidence of WNV infection to confirm the diagnosis (until WNV disease is established in Washington State).
  • For those patients who do not meet WA DOH testing criteria, testing is available at commercial laboratories or the Public Health-Seattle & King County Laboratory on a fee-for-service basis. The Public Health-Seattle & King County Laboratory offers inexpensive serum IgM testing; providers may call 206-744-8950 to arrange testing. For more information on services available, go to: www.metrokc.gov/health/lab

Tests available at the WA DOH Public Health Laboratory:

  • Enzyme immunoassay (EIA) for IgM antibody in serum of CSF.
  • Microsphere immunoassay (MIA) for IgM antibody in serum or CSF.
  • PCR assay for viral nucleic acid testing in blood or CSF (PCR is useful for evaluation of patients with immune dysfunction, but is not recommended for routine diagnosis of WNV disease because immunocompetent patients typically clear the virus shortly after symptom onset).
  • Until the disease is established in Washington State, WA DOH PHL will send all positive specimens to Centers for Disease Control and Prevention (CDC) for confirmatory testing by plaque reduction neutralization test.

Specimen collection and submission:

  • Prior to submitting specimens to WA DOH PHL, contact Public Health-Seattle & King County at 206-296-4774.

  • Submit > 1 cc of CSF and/or serum (separated serum, not whole blood) for EIA/MIA. Serum should be obtained > 8 days after onset of symptoms. A second serum specimen will be requested if the first is non-reactive or indeterminate and was obtained less than 8 days after onset of symptoms. CSF obtained less than 3 days after onset of symptoms will be accepted, however, if non-reactive, this will not rule out WNV infection, and a serum specimen obtained 8 days after onset will be requested.

  • Specimens should be refrigerated and transported cold. Frozen CSF is acceptable. Avoid repeated freeze-thaw cycles.

  • Specimens should be submitted with a completed WA DOH PHL Virus Examinations form to:

    Washington State Department of Health
    Public Health Laboratories
    1610 NE 150th St
    Shoreline, WA 98155

    For a copy of the form go to www.doh.wa.gov/EHSPHL/PHL/Forms/VirusExams.pdf or go to www.doh.wa.gov/notify/guidelines/wnv.htm and click on the link in Section D.

Treatment

  • There is no vaccine or specific therapy for WNV in humans.
  • In severe cases, intensive supportive therapy may be indicated, such as hospitalization, intravenous fluids, airway management, respiratory support, and the prevention of secondary infections.

Disease reporting guidelines

Legal reporting requirements

Health care providers and hospitals should report suspected or confirmed WNV cases to Public Health - Seattle & King County within 3 work days. Laboratories that isolate WNV or detect virus specific antibody or viral nucleic acid should report to Public Health-Seattle & King County within 2 work days. For information on how to report a suspected or confirmed case, go to: www.metrokc.gov/health/providers or call 206-296-4774.

Please report cases with any of the following:

  • Suspected or confirmed WNV neuroinvasive disease:
    Fever (in the absence of a more likely diagnosis) in a patient with at least one of the following:
    • Acute change in mental status (e.g., disorientation, obtundation, stupor, or coma), or
    • Other acute central or peripheral neurological dysfunction (e.g., paresis or paralysis, nerve palsies, sensory deficits, abnormal reflexes, seizures, or movement disorders), or
    • Cerebrospinal fluid (CSF) pleocytosis associated with an illness compatible with meningitis, or
    • WNV-specific IgM antibodies measured by any serologic testing method or detection of viral nucleic acid in CSF

  • WNV non-neuroinvasive disease:
    Fever (in the absence of a more likely diagnosis) in a patient who has laboratory evidence of WNV disease:
    • WNV-specific IgM antibodies in serum measured by any serologic testing method, or
    • Isolation of WNV from, or detection of viral nucleic acid in blood

  • Acute flaccid paralysis or presumed Guillain-Barré syndrome even in the absence of fever and other neurologic symptoms.

  • Suspected WNV disease or asymptomatic WNV infection with laboratory evidence of WNV in the following patients, even in the absence of fever and other neurologic symptoms:
    • A pregnant woman
    • A neonate or breastfeeding infant of a WNV infected mother
    • Someone who donated or received blood products in the previous month
    • Someone who donated or received a tissue or organ transplant in the previous month
    • Someone who has had occupational exposure to WNV (in a laboratory or through contact with infected animals)

  • When taking a history from a suspected WNV patient, determine if the patient received blood transfusions or organs within the 4 weeks preceding symptom onset (if so, serum or tissue samples should be retained for testing). In addition, please ask about and report any history of blood or organ donation within 2 weeks of symptom onset for persons with suspected WNV infection. Prompt reporting of these cases will facilitate follow-up including withdrawal of potentially infected blood components.

Prevention

  • Counsel patients on ways to prevent mosquito bites, especially those possibly at higher risk for severe disease such as the elderly, persons with immune deficiency, and those with diabetes. These personal protection measures include:
    • Using insect repellents containing DEET, picaridin, lemon eucalyptus oil, or IR3535.
    • Taking special care when outdoors at dusk and dawn, when mosquitoes are most active.
    • Asking families to check screen doors and windows for openings that might allow mosquitoes indoors, and repair screens if needed.
  • Advise patients to decrease standing water to the greatest extent possible, particularly around the home. Mosquitoes can breed in water that is as shallow as an inch. Ways to reduce mosquito breeding include:
    • Changing the water in bird baths and animal feeding troughs weekly
    • Emptying out children’s wading pools when not in use
    • Eliminating other sources of standing water
    • Tipping over outdoor wheelbarrows, buckets, and children’s toys when not in use so that they do not collect water

Resources

related sites

mosquitoWest Nile Virus facts

Learn what King County is doing to prevent West Nile Virus from becoming a problem.

Updated: Tuesday, June 03, 2008 at 03:08 PM

All information is general in nature and is not intended to be used as a substitute for appropriate professional advice. For more information please call 206-296-4600 (voice) or TTY Relay: 711. Mailing address: ATTN: Communications Team, Public Health - Seattle & King County, 401 5th Ave., Suite 1300, Seattle, WA 98104 or click here to email us. Because of confidentiality concerns, questions regarding client health issues cannot be responded to by e-mail. Click here for the Notice of Privacy Practices. For more information, contact the Public Health Privacy Office at 206-205-5975.

King County | Public Health | News | Services | Comments | Search

Links to external sites do not constitute endorsements by King County.
By visiting this and other King County web pages, you expressly agree to be bound by terms
and conditions of the site. The details.