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Repeat: [AAAT] = bottom strand (commonly used); [ATTT] = GenBank
top strand
Reported Primers | Ref. | PCR Primer Sequences |
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5'-GGGATTTCCCTATGGATTGG-3' (ATTT strand) 5'-GCGAAAGAATGAGACTACAT-3' (TAAA strand) |
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5'-GGAAGATGGAGTGGCTGTTA-3' (ATTT strand) 5'-CTCCAGCCTGGCGAAAGAAT-3' (TAAA strand) |
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5'-GCTTGTTAATTCATGTAGGGAAGGC-3' (ATTT strand) 5'-GTAGTCCCAGCTACTTGGCTACTC-3' (TAAA strand) |
PCR Product Sizes of Observed Alleles
(Repeat #) |
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7 | 206 bp | 138 bp | 222 bp | [ATTT]7 | 138 |
7a* | 206 bp | 138 bp | 222 bp | [ATTT]7 | 138 |
8 | 210 bp | 142 bp | 226 bp | [ATTT]8 | 5 |
9 | 214 bp | 146 bp | 230 bp | [ATTT]9 | 5 |
10 | 218 bp | 150 bp | 234 bp | [ATTT]10 | 5 |
10a* | 218 bp | 150 bp | 234 bp | [ATTT]10 | 34 |
11 | 222 bp | 154 bp | 238 bp | [ATTT]11 | 5 |
11a* | 222 bp | 154 bp | 238 bp | [ATTT]11 | 34 |
12 | 226 bp | 158 bp | 242 bp | [ATTT]12 | 5 |
12a* | 226 bp | 158 bp | 242 bp | [ATTT]12 | 391 |
13 | 230 bp | 162 bp | 246 bp | [ATTT]13 | 5 |
13a* | 230 bp | 162 bp | 246 bp | [ATTT]13 | 381 |
14 | 234 bp | 166 bp | 250 bp | [ATTT]14 | 5 |
15 | 238 bp | 170 bp | 252 bp | [ATTT]15 | 138 |
*microvariants; an A to C transversion at base 34 of the 5' flanking sequence when compared to the regular allele
Allelic Ladders: Commercially available from Promega
Common Multiplexes: FFFL; British Quadruplex