TPOX Variants


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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

4

207.18

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

4 [2] 214.05 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville, TN Re-extracted observed in multiple samples    
4 [3] 213.89 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville, TN Re-extracted observed in multiple samples    

5

212.4

ABI 310

PR, CV

Margaret Kline

Re-amplified with two kits

 

1 in 600 samples

5 [2]

214.21

ABI 377

CO

Chad Hainley

Re-amplified

Convicted offender known

1

5 [3] 218.07 - 218.09 ABI 310 ID Andrea Verzeletti, Istituto de  Medicina Legale, Universita degli Studi di Brescia, Brescia, Italia Re-extracted and Re-amplified Paternity testing case  

5 (<6)

210.96

ABI 310

PP

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

5 (<6) [2]

210.91

ABI 3100

CO

Nicole Hahn

M U F S C

Re-amplified for confirmation

 

1

5 (<6) [3]

211.04

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen - No verification

 

2

5 (<6) [4]

211 ABI 3100 CO

Nicole Hahn

M U F S C

     
5 (<6) [5] 211.14 ABI 3100 CO Nicole Hoffman     M U F S C Previously Seen - No verification   3
5 (<6) [6] 217.85 217.89 ABI 3130XL ID Laurent Cardin & Frederic G. Brard, IRCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France re-extraction and re-amplification FTA card - saliva 2 in 25000
7.1 227.05 ABI 310 ID Janel Smith, Phoenix Police Department Present in both exemplar and seven intimate evidence samples    
7.2 227.6 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN

 

Observed in multiple samples    

7.3

225.67

ABI 377

PS

Catherine Allor

Re-amplified and Reanalyzed

Paternity samples only

1 in 33300

7.3 [2] 228.69 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville  TN      

10.1

278.08

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

 

10.1 [2] 238.51-238.64 ABI 3130XL ID Laurent Cardin and Frederic G. Brard, IRCGN, France re-extraction and re-amplification FTA card - saliva/POP4 1/50.000

10.3

 

 

PP  & PP 2.1

MD Sate Police

Re-extracted, re-amplified, re-run and sequenced

Sample is actually 1 bp del. 157 bases from the repeat.

 

11

 

 

PP1.1 & ID

MD Sate Police

Re-extracted, re-amplified, re-run and sequenced

Sample is actually 1 bp del. 157 bases from the repeat

 

13.1

250.96

ABI 377

CO

Marie Samples

Present in both exemplar and evidence samples

 

 

>13 (14) 246.79 AI 3100 CO Nicole Hahn          M U F S C      

>13

257.48

ABI 3100

ID

Angela Withrow    M U F S C

Re-amplification

 

1 in 11,512

14

250.77

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

14 [2]

250.93

ABI 377

CO

Chad Hainley

Re-amplified

Convicted offender known

1

14 [3] 246.79 ABI 3100 CO KB                       M U F S C      
14 [4] 253.67 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    
14 (>13) 246.78 ABI 3100 CO

Nicole Hahn

M U F S C

Previously Seen � no verification

  2
14 (>13) [2] 246.95 ABI 3100 CO Nicole Hoffman                     M U F S C      

15 (>13)

297.01

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

3

15 (>13) [2] 250.86 abi 3100 CO Nicole Hahn          M U F S C      

15

250.88

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

15 [2] 257.78 ABI 310 ID Johnson_E USACIL Re-extracted and re-amplified Reference Blood  
15 [3] 257.74

257.78

ABI 3100 AVANT ID Julie M. Marschner, Las Vegas Metrophoitan PD Forensic Laboratory Re-amplification of reference standard 4 casework samples with matching reference standard of victim 1 (5 times in one case)

16

261.68

ABI 3130

ID/CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

16 (>13)

300.94

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

1 in 33739

16 reported variants as of 08/11/2008

 

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

6, 8, 10

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extracted 

Convicted offender

1 in 39,000

6, 8, 10 [2]

ABI 3100

ID

Misty Marra           M U F S C

Not verified/confirmed

Alabama Department of Forensic Science Convicted Offender

1 in 7840

6, 8, 10 [3]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

6, 8, 10 [4] ABI 3130xl ID Monica Price, WA Sate Patrol Crime Lab Re-extracted Convicted Offender  

6, 9, 10

ABI 377

CO

Kelly Solis    Texas DPS

Re-extracted 

Convicted offender

1 in 39,000

6, 9, 10 [2]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

6, 9, 10 [3]

ABI 3100

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

6=9=10

 

6, 9, 10 [4]

ABI 3100

ID

Paul Mazur

Eric County CPS Forensic Lab

Buffalo, NY

Multiple sample amplifications

Casework samples

 

6, 9, 10 [5]

ABI 3100

CO

Nicole Hahn  

M U F S C

Re-amplified x 1

 

1

6, 10, 11

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extracted

Convicted offender

1 in 39,000

6, 10, 11 [2]

ABI 3100

ID

Laura Kuyper

M U F S C

None

AL Dept of Forensic Sciences Convicted Offender Sample

3 in 15595

6, 10, 11 [3]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

6, 10, 11 [4] ABI 3100 CO Nicole Hoffman       M U F S C No verification - previously seen   4

6,10,12

ABI 310

CO

Diane Hanson Florida Dept of Law Enforcement

Re-extracted, observed in known and question samples

 

 

7, 8, 10

ABI 310

PP  & ID

Angelo ABATI, Belgium

2 extractions and 2 reun in PP 16 and 2 runs in ID

 

1 in 1100

7, 8, 10 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

7, 9, 10

ABI 3100

CO

Neil Quigley Molecular Pathology Laboratory Network, encode Paternity Systems

Repeated DNA isolation from original buccal cells sample, and re-ran Cofiler analysis

Routine paternity test

l in about 20,000

7, 10, 11

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

7, 10, 11 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 9, 10

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

8, 9, 10 [2]

ABI 377

ABI 310

CO

William Stubbs WA Sate Patrol

Re-extracted and amplified

Convicted offender

 

2 in 30,000

8, 9, 10 [3]

ABI 310

ID

Mark Kalinowsk

In both questioned samples and known sample

 

1

8, 9, 10 [4]

FMBIO

PP

Dayse Silva  State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 1668

8, 9, 10 [5]

ABI 3100

ID

Angela Withrow

M U F S C

Not repeated - TPOX tri-alleles very common in Alabama

Alabama Department or Forensic Science Convicted Offender

4 in 11753

8, 9, 10 [6]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

8, 9, 10  [7]

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

8, 9, 10  [8]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 9, 10 [9]

ABI 3100 AVANT

ID

Cintia Fridman, USP,Sao Paulo, Brazil

Re-amplification

Missing Children Data Base

1

8, 9, 10 [10] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
8, 9, 10 [11] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
8, 9, 10 [12] Manual Promega CTT Laboratorio de Estudios Geneticos Aplicados - Universidad Nacional de Qualimes - Argentina Re-amplified and re-analyzed   1 in 1325
8, 9, 10 [13] ABI 310 PP Melissa Martinenza

Deleware Office of the Chief Medical Examiner, DNA Unit

Re-extracted and re-amplified   2
8, 9, 10 [14] ABI 310 C) Hal Johnson, Illinois Sate Police Samples Re-amplification Observed in suspect standard  
8, 9, 11 ABI 3100 PR, ID Chun Byung-Won, NISI, Seoul, Republic of Korea Re-extraction and re-amplification Casework sample  

8, 10, 11

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extraction

Convicted offender

1 in 39,000

8, 10, 11 [2]

ABI 310

CO

Patricia BencivengaFlorida Dept of Law Enforcement

Re-extracted, present in both known and question samples

Distruction/vandalisn offenses

 

8, 10, 11 [3]

ABI 310

PP  & ID

CintiaAlvesI PATIMUP, Porto Portugal

Re-extracted and re-amplified

Mozambique sample

1 in 145

8, 10, 11 [4]

ABI 3100

ID

Laura Kuyper        M U F S C

Not verified

Alabama Department of Forensic Science Convicted Offender

1 in 8400

8, 10, 11 [5]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 417

8, 10, 11 [6]

ABI 3100

ID

Angela Withrow

M U F S C

Not verified

Alabama Department of Forensic Science Convicted Offender

2 in 12,293

8, 10, 11 [7]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian Mother has genotype 9/10/11, child has 8/10/11

1 in 25154

8, 10, 11 [8]

ABI 3100

ID

Mark Kalinowski, Erie County Central Police Services Forensic Lab, NY

 

Both in questioned and known samples

1

8, 10, 11 [9]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 10, 11 [10]

ABI 3100

CO

Misty Marra

M U F S C

Re-amplification

 

2 in 16,579

8, 10, 11 [11]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

4

8, 10, 11 [12]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

4

8, 10, 11 [13]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

5

8, 10, 11 [14]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously documented in our lab – not repeated

 

6

8, 10, 11 [15]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

8

8, 10, 11 [16]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

8

8, 10, 11 [17]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

10

8, 10, 11 [18]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

11

8, 10, 11 [19] ABI 310 CO Katrina Gomez, Illinois State Police   Observed in vaginal swab F1,F2 plus victim standard  
8, 10, 11 [20] ABI 3130 ID Yue Wang, Center for Medical Genetics, Houston TX Re-extracted and re-amplified Maternity Sample 1

8, 10, 12

ABI 377

CO

Chris Piwonka Texas DPS

Re-extracted

Convicted offender

1 in 39,000

8, 10, 12 [2]

ABI 310

PP  & ID

CintiaAlves IPATIMUP, Porto Portugal

Re-extracted and re-amplified

Mozambique sample

1 in 145

8, 10, 12 [3]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 556

8, 10, 12 [4]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

8, 11, 12

ABI 3100

PS & CO

Misty Marra               M U F S C

Re-amplification

West Virginia Convicted Offender

1 in 10613

8, 11, 12 [2]

Manual

Promega Silver Staining Kit

David Baumgarten

American Red Cross

Re-extraction and re-amplification

 

2 in 1350

8, 11, 12 [3] ABI 3100 PP ICMP Re-extraction and amplification Type 1 l in 11000

9, 10, 11

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

9, 10, 11 [2]

ABI 377

CO

Marie Samples

Present in both evidence and exemplar sample

 

 

9, 10, 11 [3]

ABI  310

CO

Christine Moraczewski Nebraska State Patrol Crime Lab

Re-extracted

Convicted Offender (known)

1

9, 10, 11 [4]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 834

9, 10, 11 [5]

ABI 3100

 

Angela Withrow

M U F S C

None (very common in Alabama)

Alabama Division of Forensic Science Convicted Offender

3 in 13913

9, 10, 11 [6]

ABI 311

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasians. Mother has genotype 9/10/11, child has 8/10/11

1 in 25254

9, 10, 11 [7]

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

9, 10, 11 [8]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

9, 10, 11 [9] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
9, 10, 11, [10] ABI 3100 CO Nicole Hahn             

M U F S C

Seen previously in our lab - No verification   4
9, 10, 11, [11] ABI 3100 CO Nicole Hoffman     

M U F S C

Previously seen - No verification   6

9, 10, 12

ABI 377

CO

Shantel Kaster Texas DPS

Re-amplified

convicted offender

1 in at least 20,000

9, 10, 12 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

10, 10, 11 ABI 3130 CO Garon Foster, Bexar County Criminal Investigation Laboratory, San Antonio  TX Multiple samples including known standard Tri-Allelic pattern, double dose of allele 10  

10, 11, 12

ABI 377

ABI 310

CO

William Stubbs WA Sate Patrol

Re-extracted and amplified

Convicted offender

 

1 in 30,000

10, 11, 12 [2]

ABI 3100 Avant

ID

John C. Barron, Richland County Sheriff's Dept, Columbia  SC

Re-amp and Re-run

 

1

10, 11, 12 [3]

ABI 310

PP

Brandt Cassidy DNA Solutions

Re-extract and Re-Amp

Paternity testing - African-American

1 in 1452

10, 11, 12 [4]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

16 reported tri-allelic patterns as of 08/11/2008

 

* Use the following abbreviations for amplification kit names:

 

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