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Microbiology Laboratory Guidebook
The Guidebook contains current protocols for analytical tests required by FSIS regulatory activities on meat, poultry and egg products.1 Specifically, microbiological methods are presented for sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the detection of extraneous materials and antimicrobial residues. Media and reagent formulations, and Most Probable Number Tables are contained in an appendix.

Methods Available On This Site:
Cover, Foreword, General Considerations (PDF Only)
1. Sample Preparation For Meat, Poultry and Pasteurized Egg Products (1998; PDF Only)
2. Physical Examination of Meat and Poultry Products (1998; PDF Only)
3. Examination of Fresh, Refrigerated and Frozen Prepared Meat, Poultry and Pasteurized Egg Products (1998; PDF Only)
4.04 Isolation and Identification of Salmonella from Meat, Poultry, and Egg Products (Feb 4, 2008; PDF Only)
4C.02 FSIS Procedure for the Use of a Polymerase Chain Reaction (PCR) Assay for Screening Salmonella in Raw Meat, Carcass Sponge Samples, Whole Bird Rinses, Ready-To-Eat Meat and Poultry Products, and Pasteurized Egg Products (Jul 13, 2007; PDF Only)
5A.01 FSIS Procedure for the Use of Escherichia coli O157:H7 Screening Tests (Jan 28, 2008; PDF Only)
5.04 Detection, Isolation and Identification of Escherichia coli O157:H7 from Meat Products (Jan 28, 2008; PDF Only)
6. Isolation, Identification, and Enumeration of Campylobacter jejuni/coli from Meat and Poultry Products (1998; PDF Only)
7. Isolation and Identification of Aeromonas Species from Meat and Poultry Products (1998; PDF Only)
8.06 Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, Egg, and Environmental Samples (Feb 19, 2008; PDF Only)
8A.03 FSIS Procedure for the Use of a Listeria monocytogenes Polymerase Chain Reaction (PCR) Screening Test (Feb 19, 2008; PDF Only)
9. Isolation & Identification of Pathogenic Yersinia enterocolitica from Meat and Poultry Products (1998; PDF Only)
10. Examination of Heat Processed, Hermetically Sealed (Canned) Meat and Poultry Products (1998; PDF Only)
11. Tests For Enzymes In Meat and Meat Products (1998; PDF Only)
12. Examination of Meat and Poultry Products For Bacillus cereus (1998; PDF Only)
13. Examination of Meat and Poultry Products For Clostridium perfringens (1998; PDF Only)
14. Methods For The Detection of Clostridium botulinum Toxins In Meat and Poultry Products (1998; PDF Only)
16. Agarose Thin-Layer Isoelectric Focusing (TLIEF) For Species Determination of Raw Muscle Tissues (1998; PDF Only)
17.02 Identification of Animal Species in Meat and Poultry Products (Feb 10, 2005; PDF Only)
18. Species Identification Field Tests (SIFT) (1998; PDF Only)
19. Competitive Enzyme-Linked Immunoassay (CELIA) For The Detection and Quantitation of Chloramphenicol (1998; PDF Only)
Chapter Numbers 22-31 Reserved For Future Use.
32.01 Detection and Identification of Extraneous Material In Meat and Poultry Products - Revised (Dec 30, 2004; PDF Only)
Part A Detection of Light Filth In Prepared Infant Foods Containing Meat and Poultry
Part B Detection and Identification of Extraneous Materials In Non-Meat Food Ingredients - Macroscopic Examination
Part C Detection of Glass and Non-Aluminum Metals In Meat and Poultry Products
Part D Method For The Isolation of Glass from Prepared Meat and Poultry Baby Foods
Part E Analysis of Fecal Material
33. Detection of Antimicrobial Residues In Meat and Poultry Tissue By Screen Tests (1998; PDF Only)
Part A Detection of Antimicrobial Residues By Swab Test On Premises (STOP)
Part B Detection of Antimicrobial Residue In Calves By Calf Antibiotic and Sulfonamide Test (CAST)
Part C Tentative Confirmation of CAST Results For Sulfonamide Residues In Meat and Poultry Tissue
Part D Detection of Antimicrobial Residue By Fast Antimicrobial Screen Test (FAST)
Part E Evaluation of Antimicrobial Residues In Meat and Poultry Tissue By A Modified Fast Antimicrobial Screen Test (M-FAST)
34.02 Bioassay For The Detection, Identification and Quantitation of Antimicrobial Residues In Meat and Poultry Tissue (May 2, 2007; PDF Only)
35. Detection of Antibiotic and Sulfonamide Residues In Meat Tissue By Commercially Available Immunoassay Kits (1998; PDF Only)
40.00 Avian Influenza Detection in the Chicken Heart Using Real-Time Reverse Transcriptase PCR (Oct 8, 2007; PDF Only)
Appendices:
1.03 Media and Reagents (Jan 28, 2008; PDF Only)
2.03 Most Probable Number Procedure and Tables (Jan 28, 2008; PDF Only)
1 The methods described in this guidebook are for use by the FSIS laboratories. FSIS does not specifically endorse any of the mentioned test products and acknowledges that equivalent products may be available for laboratory use.


Last Modified: February 6, 2008

 

 

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