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coagulation factor XIII b subunit gene |
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Repeat: [AAAT] = bottom strand (commonly used); [TTTA] = GenBank
top strand; sometimes even designated [ATTT] (see ref. 391)
Reported Primers | Ref. | PCR Primer Sequences |
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5'-TGAGGTGGTGTACTACCATA-3' (TTTA strand)
5'-GATCATGCCATTGCACTCTA-3' (AAAT strand) |
PCR Product Sizes of Observed Alleles
(Repeat #) |
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using top strand |
using bottom strand |
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6 | 169 bp | [TTTA]7 | [AAAT]6 | 391 |
7 | 173 bp | [TTTA]8 | [AAAT]7 | 391 |
8 | 177 bp | [TTTA]9 | [AAAT]8 | 391 |
9 | 181 bp | [TTTA]10 | [AAAT]9 | 391 |
9c* | 181 bp | [TTTA]10 | [AAAT]9 | 391 |
10 | 185 bp | [TTTA]11 | [AAAT]10 | 391 |
10c* | 185 bp | [TTTA]11 | [AAAT]10 | 391 |
11 | 189 bp | [TTTA]12 | [AAAT]11 | 391 |
11c* | 189 bp | [TTTA]12 | [AAAT]11 | 381 |
12a* | 193 bp | [TTTA]12 [TTTT] | [AAAT]11[AAAA] | 213 |
*microvariants; variant alleles with "c" designation have slower electrophoretic mobilities relative to the regular alleles (c = cathodal); 9c and 10c contain an A to C transversion in the 3' flanking region; 12a (a = anodal) contains a T to A transversion in the 12 repeat (using bottom strand designation)
Allelic Ladders: Commercially available from Promega
Common Multiplexes: FFFL