Cellular and Genetic Tracers of Transport Time in Groundwater Systems

Publisher: USGS | Science Center: Western Fisheries Research Center (WFRC, Seattle) | Format: URL

wfrc.usgs.gov — Artificially maintained water levels within the lake and stresses placed upon the aquifer by well withdrawals facilitate movement of water from the lake into the local ground water system, potentially allowing contaminants to enter the aquifer from the lake. This raises national concern because similar lakeside settings exist throughout the United More... Artificially maintained water levels within the lake and stresses placed upon the aquifer by well withdrawals facilitate movement of water from the lake into the local ground water system, potentially allowing contaminants to enter the aquifer from the lake. This raises national concern because similar lakeside settings exist throughout the United States. This research summary was established to develop a method of quantifying Groundwater Under the Direct Influence of Surface Water (GWUDISW) by identifying diatom proteins using various biochemical and immunological techniques. Diatoms from both surface and ground waters will be identified, counted, and proteins will be extracted from common diatom species and purified. These proteins will be used to develop monoclonal antibodies and accompanying enzyme immunoassays (Walker, 1996) that will facilitate the identification and quantification of the proteins in ground water and hence GWUDISW. Less...