U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M72
Koser's Citrate Broth

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NaNH4HPO4·4H2O 1.5 g
KH2PO4 (monobasic) 1 g
MgSO4·7H2O 0.2 g
Sodium citrate·2H2O 3 g
Distilled water 1 liter

Dispense into screw-cap tubes as desired. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2. This formulation is listed in Official Methods of Analysis of AOAC International, and Standard Methods for the Examination of Wastewater of the APHA. It differs from the composition of commercially available dehydrated medium. The latter is satisfactory.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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