U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M124
Plate Count Agar
(Standard Methods)

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Tryptone 5 g
Yeast extract 2.5 g
Dextrose 1 g
Agar 15 g
Distilled water 1 liter

 

Heat to dissolve ingredients. Dispense into suitable tubes or flasks. Autoclave 15 min at 121°C. Final pH, 7.0 ± 0.2.


For viable yeasts and molds, dispense 20-25 ml portions into sterile 15 x 100 mm petri dishes.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.


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Hypertext updated by rim/cjm 2005-OCT-21