U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R30
Giemsa Stain

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Giemsa stain
(Matheson Coleman & Bell, Norwood, OH 45212)		 1 g
Glycerol 66 ml
Methanol (absolute) 66 ml

Distilled stain in glycerol by heating 1.5-2.0 h at 55-60°C. Add methanol. Store stain in tightly stoppered bottle at 22°C for at least 2 weeks. Dilute stock solution with distilled water (1+9) before use.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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