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M186
Trypicase (Tryptic) Soy Broth
with ferrous sulfate
Trypticase peptone | 17 g |
Phytone peptone | 3 g |
NaCl | 5 g |
K2HPO4 | 2.5 g |
Glucose | 2.5 g |
Ferrous sulfate | 35 mg |
Distilled water | 1.0 liter |
Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
Hypertext updated by rim/cjm 2005-OCT-21