M16
Arginine-Glucose Slant
(AGS)
|
M16
Arginine-Glucose Slant
(AGS)
| Peptone | 5 g |
| Yeast extract | 3 g |
| Tryptone | 10 g |
| NaCl | 20 g |
| Glucose | 1 g |
| L-Arginine (hydrochloride) | 5 g |
| Ferric ammonium citrate | 0.5 g |
| Sodium thiosulfate | 0.3 g |
| Bromocresol purple | 0.02 g |
| Agar | 13.5 g |
| Distilled water | 1 liter |
Suspend ingredients in distilled water and boil to dissolve. Dispense into tubes (for 13 x 100 mm tubes use 5 ml). Autoclave 10-12 min at 121°C. After sterilization, solidify as slants. Final pH, 6.8-7.0.
Hypertext updated by rim/cjm 2005-OCT-21
