U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M13
Andrade's Carbohydrate Broth and Indicator

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Base


Beef extract 3 g
Peptone or gelysate 10 g
NaCl 10 g
Distilled water 1 liter

 

Adjust pH to 7.2 ± 0.2. Autoclave 15 min at 121°C.

 



Andrade's indicator

Acid fuchsin * 0.2 g
1 N NaOH 16 ml
Distilled water 84 ml

 

Allow to decolorize before use. Add 1-2 ml NaOH if necessary. Add 10 ml indicator to 1 liter base.


* Acid fuchsin is a potential carcinogen. Avoid inhalation and contact with skin.


Carbohydrate stock solution.

Prepare dextrose, lactose, sucrose, and mannitol in 10% solutions. Prepare dulcitol, salicin, and other carbohydrates in 5% solutions. Sterilize by filtration through 0.20 µm membrane. Dilute sugar solutions 1:10 in base with Andrade's indicator to give recommended concentration. Mix gently.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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