Add the 55.5 g of the first 4 components (basal medium) to 1 liter distilled water. Bring gently to boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 min. Cool to 50°C and aseptically add supplement, mix, and pour into sterile petri dishes. To prepare supplement, dissolve cycloheximide, colistin sulfate, acriflavin, cefotetan, and fosfomycin in 10 ml of 1:1 mixture of ethanol and distilled water. Filter-sterilize supplement before use. Oxford basal medium and supplement mixture are available commercially.

Hypertext updated by rim/cjm 2005-OCT-21