RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus macrocephalus
FDA Seafood List Common Name: Pacific Cod
FDA Seafood List Market Name: Cod (Alaska Cod)
Unique IEF Gel Code: 19940912gel01
Unique Web Code: pcg01Srr
Tissue Aliquot Code: gadumacr I94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	60	5.2	0.0869644	19	2.78056	19.6	13.1	3.5	60.4	19.3	0.0811097
2	4.55	215	18.7	0.135223	5	1.11422	7.9	5.2	4.55	214.8	5	0.12699
3	5.2	303	26.4	0.188314	5	1.57964	11.1	7.4	5.2	302.8	4.9	0.182633
4	5.85	457	39.8	0.28308	3	1.72634	12.2	8.1	5.85	457.1	3.4	0.284886
5	6.55	621	54.1	0.213291	4	1.46704	10.3	6.9	6.55	620.3	4	0.209531
6	6.85	660	57.5	0.0556682	7	0.613263	4.3	2.9	6.85	659.7	7.2	0.0481467
7	7.35	747	65.1	0.139728	5	1.25885	8.9	5.9	7.35	746	5.2	0.137865
8	8.15	900	78.5	0.0649571	12	1.23948	8.7	5.8	8.15	898.8	11.5	0.0605747
9	8.45	964	84	0.0972372	8	1.3603	9.6	6.4	8.45	963.6	7.9	0.0975025
10	8.65	1001	87.3	0.0885173	7	1.03531	7.3	4.9	8.65	1000.6	7.1	0.0819562

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.794	105	9.2	0.0508679	6	0.516056	2.6	2.1	3.794	104.8	6.4	0.0455643
2	4.109	152	13.3	0.336259	4	2.73654	13.9	11.3	4.109	152.1	4.4	0.349079
3	4.309	182	15.9	0.207229	4	1.4478	7.4	6	4.309	181.9	4	0.206439
4	4.476	207	18.1	0.0102123	2	0.0230725	0.1	0.1	4.476	206.5	2.2	0.00589994
5	4.639	230	20.1	0.0328184	12	0.704776	3.6	2.9	4.639	225.3	12.5	0.0318557
6	4.823	255	22.3	0.0960368	3	0.408333	2.1	1.7	4.823	254.9	2.6	0.088015
7	4.956	273	23.8	0.0653511	3	0.398123	2	1.6	4.956	272.7	3.5	0.064602
8	5.052	286	25	0.0377279	3	0.218156	1.1	0.9	5.052	285.4	3.3	0.0371693
9	5.119	295	25.8	0.0143054	8	0.209126	1.1	0.9	5.119	295.4	8.1	0.0146198
10	5.185	304	26.6	0.0455993	4	0.291002	1.5	1.2	5.185	304.7	3.7	0.0446038
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.255	319	27.9	0.288863	4	2.21548	11.2	9.2	5.255	319	4.2	0.295928
12	5.327	336	29.3	0.137929	3	0.823048	4.2	3.4	5.327	336.1	3.3	0.139134
13	5.436	362	31.6	0.104642	3	0.593615	3	2.5	5.436	362.2	3.3	0.100839
14	5.529	384	33.5	0.0129944	5	0.121793	0.6	0.5	5.529	383.7	5.4	0.0126592
15	5.576	395	34.5	0.0132229	3	0.0747561	0.4	0.3	5.576	395	3.2	0.013378
16	5.626	407	35.5	0.305995	3	1.51957	7.7	6.3	5.626	406.7	2.8	0.311403
17	5.685	421	36.8	0.164779	3	0.757461	3.8	3.1	5.685	421	2.6	0.165305
18	5.782	444	38.8	0.0203073	9	0.281079	1.4	1.2	5.782	440.1	9.1	0.0174007
19	5.833	456	39.8	0.11	3	0.493231	2.5	2	5.833	456	2.7	0.103322
20	5.916	475	41.5	0.0197424	2	0.0880295	0.4	0.4	5.916	475.4	2.4	0.0203547
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.964	486	42.4	0.02954	3	0.149131	0.8	0.6	5.964	485.5	3	0.0284221
22	5.995	493	43.1	0.0340113	5	0.33321	1.7	1.4	5.995	494.9	5.1	0.037165
23	6.039	503	43.9	0.247249	2	0.789858	4	3.3	6.039	502.7	1.8	0.244036
24	6.078	512	44.7	0.009574	2	0.0270984	0.1	0.1	6.078	512	1.6	0.00972335
25	6.148	528	46.1	0.223112	3	1.20139	6.1	5	6.148	526.9	3.4	0.200081
26	6.258	553	48.3	0.0703855	7	0.661983	3.4	2.7	6.258	553.3	7	0.0532394
27	6.377	580	50.7	0.0605186	18	1.80028	9.1	7.4	6.377	579.3	18	0.0564288
28	7.126	707	61.7	0.0338605	8	0.419202	2.1	1.7	7.126	712.3	8.5	0.0279038
29	7.371	750	65.5	0.0294058	9	0.394202	2	1.6	7.371	748.4	8.9	0.0249882

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	62	5.4	0.0804382	13	1.70811	14.4	9.1	3.5	63	12.9	0.0747558
2	4.55	221	19.3	0.143198	4	0.986234	8.3	5.3	4.55	221.1	4.4	0.125574
3	5.2	309	27	0.20302	4	1.37968	11.7	7.4	5.2	309.1	4	0.196748
4	5.85	463	40.5	0.331976	3	1.71428	14.5	9.2	5.85	463.1	2.9	0.331517
5	6.55	618	54	0.180037	4	1.35238	11.4	7.2	6.55	617.8	4.4	0.174037
6	6.85	658	57.5	0.0433096	7	0.476147	4	2.5	6.85	657.9	7.1	0.0375763
7	7.35	745	65.1	0.124356	5	1.08823	9.2	5.8	7.35	744.5	5.1	0.121198
8	8.15	896	78.3	0.0539606	12	1.07389	9.1	5.7	8.15	894.8	12	0.0506159
9	8.45	962	84.1	0.0904511	8	1.19301	10.1	6.4	8.45	961.6	7.6	0.0880814
10	8.65	1000	87.4	0.088982	6	0.867666	7.3	4.6	8.65	1000	5.9	0.0831294

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	60	5.2	0.0576139	13	1.28866	5.5	4.7	3.5	60.8	13.1	0.0554574
2	3.929	127	11.1	0.0377185	8	0.517335	2.2	1.9	3.929	128.8	8.4	0.0346103
3	4.153	162	14.2	0.352466	4	2.44196	10.5	8.8	4.153	161.9	3.8	0.358114
4	4.32	188	16.4	0.189713	4	1.29217	5.5	4.7	4.32	188.1	3.8	0.19206
5	4.55	224	19.6	0.10281	7	1.0421	4.5	3.8	4.55	223.9	6.9	0.0848901
6	4.647	237	20.7	0.0367707	5	0.308167	1.3	1.1	4.647	236.4	4.9	0.0356061
7	4.804	258	22.6	0.0107383	4	0.0662483	0.3	0.2	4.804	257.5	3.6	0.0104541
8	4.871	267	23.4	0.014129	2	0.0401943	0.2	0.1	4.871	267.3	1.7	0.0136575
9	4.946	277	24.2	0.0595286	3	0.33222	1.4	1.2	4.946	277.4	3.2	0.0577023
10	5.021	287	25.1	0.0320609	3	0.155814	0.7	0.6	5.021	287	2.8	0.0311825
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.103	298	26.1	0.0248448	5	0.227622	1	0.8	5.103	298.5	5	0.0257999
12	5.2	311	27.2	0.102436	4	0.707661	3	2.6	5.2	310.2	3.8	0.10372
13	5.259	325	28.4	0.233386	4	1.78025	7.6	6.4	5.259	324.8	4.1	0.24367
14	5.323	340	29.7	0.155851	3	0.728172	3.1	2.6	5.323	340.6	2.7	0.153786
15	5.438	367	32.1	0.0945605	3	0.540106	2.3	2	5.438	367.4	3.2	0.0941363
16	5.519	386	33.8	0.00952922	4	0.0683132	0.3	0.2	5.519	386.9	4.5	0.00863618
17	5.638	414	36.2	0.239968	3	1.43205	6.1	5.2	5.638	414.1	3.4	0.236863
18	5.701	429	37.5	0.137853	4	0.899634	3.9	3.3	5.701	428.5	3.9	0.13135
19	5.791	450	39.4	0.0146453	6	0.132383	0.6	0.5	5.791	449.3	5.7	0.0130167
20	5.85	464	40.6	0.218098	3	1.07604	4.6	3.9	5.85	463.9	2.8	0.219736
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.942	484	42.3	0.00935203	4	0.0713667	0.3	0.3	5.942	483.1	4.4	0.00908401
22	5.988	494	43.2	0.0371971	3	0.173065	0.7	0.6	5.988	493.9	3	0.0329535
23	6.025	502	43.9	0.0239201	8	0.315724	1.4	1.1	6.025	503.8	8	0.0223817
24	6.076	513	44.9	0.143214	2	0.56907	2.4	2.1	6.076	512.8	2.4	0.136014
25	6.195	539	47.2	0.14811	3	0.854343	3.7	3.1	6.195	537.6	3.3	0.144353
26	6.311	564	49.3	0.063727	6	0.495676	2.1	1.8	6.311	563.5	6	0.0466574
27	6.407	585	51.2	0.0434859	18	1.10343	4.7	4	6.407	587.4	18	0.0345863
28	6.55	616	53.9	0.0896269	6	0.787973	3.4	2.9	6.55	614.2	6	0.0746962
29	6.85	660	57.7	0.048892	6	0.471988	2	1.7	6.85	660.1	6.4	0.0413272
30	7.35	746	65.3	0.128422	6	1.36228	5.8	4.9	7.35	745.2	6.2	0.123156
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	893	78.1	0.00857976	6	0.104837	0.4	0.4	8.15	894.3	6.2	0.0095273
32	8.45	965	84.4	0.0726256	9	1.13951	4.9	4.1	8.45	964.3	9	0.0715348
33	8.65	1004	87.8	0.0633079	7	0.801055	3.4	2.9	8.65	1003.8	7	0.0649957

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.878	112	9.8	0.0241027	13	0.523741	3.2	2.7	3.878	114.3	13.3	0.0222559
2	4.143	153	13.4	0.305487	5	2.46419	15	12.8	4.143	153	4.5	0.307341
3	4.311	179	15.7	0.163649	4	1.2251	7.4	6.4	4.311	179.4	4.2	0.163931
4	4.617	225	19.7	0.0281634	13	0.575166	3.5	3	4.617	221.5	12.8	0.0253247
5	4.817	252	22.1	0.00596112	1	0.0109082	0.1	0.1	4.817	252.2	1.3	0.004684
6	4.899	263	23	0.00998752	4	0.0584264	0.4	0.3	4.899	263.8	3.6	0.00920233
7	4.996	276	24.2	0.00721384	3	0.0380644	0.2	0.2	4.996	275	2.9	0.00752527
8	5.07	286	25.1	0.00822727	5	0.0688744	0.4	0.4	5.07	286.2	4.9	0.00788492
9	5.174	300	26.3	0.0118643	3	0.0536786	0.3	0.3	5.174	298.6	2.9	0.0103157
10	5.245	314	27.5	0.139721	6	1.31587	8	6.9	5.245	314.8	6.1	0.122034
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.343	337	29.5	0.0500101	12	1.35047	8.2	7	5.343	328.8	12.1	0.0630701
12	5.433	358	31.4	0.0433888	7	0.52975	3.2	2.8	5.433	361.9	7	0.0429984
13	5.536	382	33.5	0.0107027	7	0.120092	0.7	0.6	5.536	380.8	7.3	0.0092626
14	5.596	396	34.7	0.000583239	1	0	0	0	5.596	393	1	0
15	5.638	406	35.6	0.129932	4	0.598681	3.6	3.1	5.638	406.3	3.6	0.0949903
16	5.694	419	36.7	0.0791927	13	1.80067	10.9	9.4	5.694	417.7	13.1	0.0778324
17	5.861	458	40.1	0.0409563	6	0.414136	2.5	2.2	5.861	457.8	5.9	0.0397648
18	5.943	477	41.8	0.000721887	1	0.00586508	0	0	5.943	478.3	0.9	0.00365511
19	5.994	489	42.9	0.0200093	9	0.318391	1.9	1.7	5.994	492	9.5	0.0189196
20	6.033	498	43.6	0.0223628	6	0.0057422	0	0	6.033	495.6	5.8	0.000558992
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.059	504	44.2	0.105874	4	0.657809	4	3.4	6.059	504.9	3.6	0.104539
22	6.106	515	45.1	0.00988713	4	0.0507466	0.3	0.3	6.106	517.6	3.9	0.00726833
23	6.154	526	46.1	0.0754814	4	0.688573	4.2	3.6	6.154	528.2	4	0.0965631
24	6.171	530	46.5	0.13155	3	0.368547	2.2	1.9	6.171	531.8	2.6	0.0803026
25	6.296	559	49	0.0684565	8	0.952328	5.8	5	6.296	559.7	8.1	0.0663101
26	6.378	578	50.7	0.0506692	10	0.708834	4.3	3.7	6.378	578.6	9.6	0.0417498
27	6.468	599	52.5	0.0458435	13	0.922487	5.6	4.8	6.468	599.7	12.6	0.0412001
28	7.081	700	61.3	0.013494	3	0.0587725	0.4	0.3	7.081	700.6	2.8	0.0118251
29	7.162	714	62.6	0.0241242	9	0.340409	2.1	1.8	7.162	715.6	8.7	0.0221965
30	7.401	756	66.3	0.0145247	12	0.220576	1.3	1.1	7.401	752.9	12.2	0.0102012

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.2	0.125519	14	2.77939	20.2	13.8	3.5	47.8	13.7	0.114571
2	4.55	209	18.3	0.124496	6	1.17321	8.5	5.8	4.55	208.3	5.5	0.119287
3	5.2	297	26.1	0.16282	5	1.51497	11	7.5	5.2	296.9	5.2	0.162965
4	5.85	448	39.3	0.254404	4	1.75468	12.8	8.7	5.85	447.8	3.8	0.259124
5	6.55	621	54.5	0.206092	4	1.47138	10.7	7.3	6.55	621.3	4.2	0.199614
6	6.85	661	58	0.0521106	7	0.517455	3.8	2.6	6.85	660.3	6.7	0.0435494
7	7.35	748	65.6	0.150461	6	1.45525	10.6	7.2	7.35	746.8	5.6	0.14578
8	8.15	898	78.8	0.0665226	11	1.15224	8.4	5.7	8.15	899.4	10.8	0.060022
9	8.45	966	84.7	0.0903057	7	1.08264	7.9	5.4	8.45	966	6.8	0.0893897
10	8.65	1004	88.1	0.0870328	6	0.842369	6.1	4.2	8.65	1003.7	5.6	0.0855518

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 122

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 19.600 4.550 7.900 5.200 11.100 5.850 12.200 6.550 10.300 6.850 4.300 7.350 8.900 8.150 8.700 8.450 9.600 8.650 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 10 Return to Lane Navigator
3.794 2.600 4.109 13.900 4.309 7.400 4.476 0.100 4.639 3.600 4.823 2.100 4.956 2.000 5.052 1.100 5.119 1.100 5.185 1.500 5.255 11.200 5.327 4.200 5.436 3.000 5.529 0.600 5.576 0.400 5.626 7.700 5.685 3.800 5.782 1.400 5.833 2.500 5.916 0.400 5.964 0.800 5.995 1.700 6.039 4.000 6.078 0.100 6.148 6.100 6.258 3.400 6.377 9.100 7.126 2.100 7.371 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 29 Return to Lane Navigator
3.500 14.400 4.550 8.300 5.200 11.700 5.850 14.500 6.550 11.400 6.850 4.000 7.350 9.200 8.150 9.100 8.450 10.100 8.650 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 10 Return to Lane Navigator
3.500 5.500 3.929 2.200 4.153 10.500 4.320 5.500 4.550 4.500 4.647 1.300 4.804 0.300 4.871 0.200 4.946 1.400 5.021 0.700 5.103 1.000 5.200 3.000 5.259 7.600 5.323 3.100 5.438 2.300 5.519 0.300 5.638 6.100 5.701 3.900 5.791 0.600 5.850 4.600 5.942 0.300 5.988 0.700 6.025 1.400 6.076 2.400 6.195 3.700 6.311 2.100 6.407 4.700 6.550 3.400 6.850 2.000 7.350 5.800 8.150 0.400 8.450 4.900 8.650 3.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 33 Return to Lane Navigator
3.878 3.200 4.143 15.000 4.311 7.400 4.617 3.500 4.817 0.100 4.899 0.400 4.996 0.200 5.070 0.400 5.174 0.300 5.245 8.000 5.343 8.200 5.433 3.200 5.536 0.700 5.596 0 5.638 3.600 5.694 10.900 5.861 2.500 5.943 0 5.994 1.900 6.033 0 6.059 4.000 6.106 0.300 6.154 4.200 6.171 2.200 6.296 5.800 6.378 4.300 6.468 5.600 7.081 0.400 7.162 2.100 7.401 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 30 Return to Lane Navigator
3.500 20.200 4.550 8.500 5.200 11.000 5.850 12.800 6.550 10.700 6.850 3.800 7.350 10.600 8.150 8.400 8.450 7.900 8.650 6.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.