RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Great Barracuda ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sphyraena barracuda
FDA Seafood List Common Name: Great Barracuda
FDA Seafood List Market Name: Barracuda
Unique IEF Gel Code: 19960103gel03
Unique Web Code: gbg01Srr
Tissue Aliquot Code: sphybarr L95-002 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.8	0.168311	17	5.16186	15.2	11.8	3.5	44.9	16.8	0.173856
2	4.55	179	16.2	0.242234	5	2.18093	6.4	5	4.55	179.7	5.2	0.238426
3	5.2	264	23.8	0.314092	5	2.87644	8.5	6.6	5.2	263.8	5.2	0.315106
4	5.85	425	38.4	0.612009	3	3.27895	9.7	7.5	5.85	425.5	3	0.625624
5	6.55	593	53.6	0.374822	5	2.77455	8.2	6.3	6.55	592.1	4.6	0.343044
6	6.85	632	57.1	0.168658	7	1.78646	5.3	4.1	6.85	632.8	6.9	0.146048
7	7.35	723	65.3	0.411276	7	4.68795	13.8	10.7	7.35	721.5	7.1	0.371653
8	8.15	879	79.4	0.112787	7	1.46532	4.3	3.3	8.15	879.7	7.5	0.110691
9	8.45	938	84.7	0.197324	5	1.80523	5.3	4.1	8.45	938.2	5.2	0.194596
10	8.65	976	88.2	0.189636	5	1.78587	5.3	4.1	8.65	975.8	5.4	0.18525
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1089	98.4	0.552106	7	6.17163	18.2	14.1	9.3	1087.5	7	0.497817

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.86	91	8.2	0.947733	5	9.04371	17.6	9.9	3.86	91.4	5.1	0.99615
2	4.658	193	17.4	1.02314	4	8.78747	17.1	9.6	4.658	192.4	4.5	1.10266
3	4.912	226	20.4	0.122498	4	0.613587	1.2	0.7	4.912	225.2	3.5	0.0987777
4	5.218	268	24.2	0.0867719	7	0.961204	1.9	1.1	5.218	267.8	7.5	0.0723766
5	5.336	297	26.8	0.16483	2	0.561457	1.1	0.6	5.336	297	2	0.161334
6	5.631	370	33.4	0.393843	9	4.56423	8.9	5	5.631	370.6	8.9	0.289853
7	5.729	394	35.6	1.08587	4	7.48528	14.6	8.2	5.729	395.1	4.1	1.02229
8	5.822	417	37.7	0.393972	11	5.80722	11.3	6.4	5.822	414.9	10.6	0.309343
9	5.879	431	38.9	0.0525724	1	0	0	0	5.879	426	1	0
10	6	460	41.6	0.0709606	8	0.734052	1.4	0.8	6	456.2	7.9	0.0522173
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.364	547	49.4	0.0891884	3	0.417678	0.8	0.5	6.364	546.6	2.7	0.0887766
12	6.523	585	52.8	0.224498	14	4.67533	9.1	5.1	6.523	583.9	14.4	0.183124
13	6.681	609	55	0.703215	5	6.02607	11.7	6.6	6.681	610.6	5.3	0.640012
14	7.729	798	72.1	0.191221	5	1.71605	3.3	1.9	7.729	797.3	5.1	0.190874

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.3	0.202748	18	6.38073	16.2	12.5	3.5	48.3	17.6	0.204193
2	4.55	179	16.2	0.384603	4	2.55924	6.5	5	4.55	179.3	4.2	0.342103
3	5.2	263	23.7	0.496229	4	2.99651	7.6	5.9	5.2	262.6	3.5	0.478909
4	5.85	423	38.2	0.659804	3	3.72569	9.5	7.3	5.85	423.5	3.1	0.680862
5	6.55	590	53.2	0.419461	5	3.10961	7.9	6.1	6.55	589.6	4.5	0.388077
6	6.85	631	56.9	0.202098	7	2.18013	5.5	4.3	6.85	631	7.1	0.173677
7	7.35	723	65.3	0.489629	7	5.15039	13.1	10.1	7.35	722.1	6.6	0.44344
8	8.15	884	79.8	0.117902	7	1.46855	3.7	2.9	8.15	883.5	7.3	0.112821
9	8.45	940	84.8	0.226535	5	2.03386	5.2	4	8.45	940.4	5.1	0.223736
10	8.65	977	88.2	0.201311	5	1.8301	4.7	3.6	8.65	976.2	5.2	0.197411
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1090	98.4	0.653792	7	7.92072	20.1	15.6	9.3	1088.5	7.4	0.60521

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	41	3.7	0.17053	18	5.33956	5.2	4.6	3.5	43.2	18.2	0.165967
2	3.848	91	8.2	0.946965	5	9.27111	9	8	3.848	90.9	5.3	0.98348
3	4.55	192	17.3	1.01155	5	9.04719	8.8	7.8	4.55	192.5	4.7	1.07641
4	4.849	226	20.4	0.137415	4	0.772144	0.8	0.7	4.849	224.9	3.9	0.110697
5	5.2	266	24	0.12652	4	0.911835	0.9	0.8	5.2	266.4	4.2	0.123565
6	5.236	275	24.8	0.307045	5	2.33567	2.3	2	5.236	275.3	4.5	0.291895
7	5.319	296	26.7	0.173779	3	0.767423	0.7	0.7	5.319	296.3	2.8	0.153064
8	5.604	368	33.2	0.323969	6	2.77105	2.7	2.4	5.604	367.7	5.5	0.283015
9	5.644	378	34.1	0.0968897	2	0.424306	0.4	0.4	5.644	377.3	2.4	0.100326
10	5.703	393	35.5	1.00225	4	7.33657	7.2	6.3	5.703	393.5	4.3	0.953298
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.795	416	37.5	0.421397	10	6.31188	6.2	5.5	5.795	414.4	10.1	0.351498
12	5.85	430	38.8	0.42426	3	1.94667	1.9	1.7	5.85	430.6	2.7	0.407026
13	5.985	460	41.5	0.0431196	7	0.566685	0.6	0.5	5.985	455.5	6.7	0.0479177
14	6.371	546	49.3	0.0962375	3	0.476471	0.5	0.4	6.371	546.3	2.8	0.0963286
15	6.55	586	52.9	0.193923	14	4.22464	4.1	3.7	6.55	581.1	13.7	0.174552
16	6.731	612	55.2	0.665486	4	4.05018	4	3.5	6.731	612.9	3.9	0.592647
17	6.85	629	56.8	0.136746	12	2.97239	2.9	2.6	6.85	622.1	12.4	0.135148
18	7.35	721	65.1	0.446348	5	3.7208	3.6	3.2	7.35	720.5	5	0.420673
19	7.773	804	72.6	0.204806	5	1.83269	1.8	1.6	7.773	804.4	5.3	0.196411
20	8.15	878	79.2	0.193707	14	4.22046	4.1	3.7	8.15	877.4	14.3	0.166595
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	940	84.8	0.227679	15	5.13519	5	4.4	8.45	945.1	15.4	0.188029
22	8.65	973	87.8	0.529295	19	25.3706	24.8	22	8.65	985.5	18.7	0.765686
23	9.3	1091	98.5	0.38454	4	2.67203	2.6	2.3	9.3	1090.4	3.9	0.391289

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.812	85	7.7	0.929738	5	8.58436	17.3	9.1	3.812	84.7	5	0.968451
2	4.55	183	16.5	0.963373	5	8.49226	17.2	9	4.55	182.5	4.8	1.00378
3	4.808	214	19.3	0.0693983	4	0.460816	0.9	0.5	4.808	213.8	3.9	0.067458
4	5.142	254	22.9	0.0576487	8	0.855136	1.7	0.9	5.142	254.6	8.3	0.0579704
5	5.295	285	25.7	0.0623771	5	0.537735	1.1	0.6	5.295	284.6	4.5	0.0669024
6	5.59	359	32.4	0.278424	10	4.10748	8.3	4.4	5.59	360.5	10.1	0.228332
7	5.689	384	34.7	1.01202	5	7.84149	15.8	8.3	5.689	384.3	4.6	0.965114
8	5.78	407	36.7	0.353209	9	4.66078	9.4	4.9	5.78	405.1	8.9	0.297111
9	5.84	422	38.1	0.047577	1	0	0	0	5.84	416	1	0
10	5.972	453	40.9	0.0271986	9	0.701014	1.4	0.7	5.972	446.9	9	0.0439662
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.349	541	48.8	0.0918969	2	0.398899	0.8	0.4	6.349	540.7	2.5	0.0904252
12	6.511	579	52.3	0.218804	16	5.25938	10.6	5.6	6.511	579.4	15.8	0.187732
13	6.68	606	54.7	0.741251	5	6.04328	12.2	6.4	6.68	607.7	5.4	0.632623
14	7.727	797	71.9	0.182639	5	1.56259	3.2	1.7	7.727	796.9	4.8	0.182065

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	46	4.1	0.191861	18	6.14781	15.2	11.6	3.5	44.6	17.8	0.194989
2	4.55	174	15.7	0.293647	5	2.62925	6.5	5	4.55	174.1	5.2	0.283755
3	5.2	256	23.1	0.356594	5	3.23258	8	6.1	5.2	256.3	5.1	0.356948
4	5.85	419	37.8	0.61769	4	3.88527	9.6	7.3	5.85	418.7	3.5	0.621458
5	6.55	590	53.2	0.471825	4	3.36819	8.3	6.4	6.55	589	4.3	0.437093
6	6.85	630	56.8	0.206079	7	2.17004	5.4	4.1	6.85	629.7	6.9	0.176383
7	7.35	724	65.3	0.617222	6	6.55919	16.2	12.4	7.35	723.4	6.5	0.570225
8	8.15	883	79.6	0.11971	7	1.49763	3.7	2.8	8.15	883.3	7.2	0.117041
9	8.45	940	84.8	0.229187	6	2.22336	5.5	4.2	8.45	939.8	5.6	0.223827
10	8.65	975	87.9	0.201595	5	1.89281	4.7	3.6	8.65	974.9	5.5	0.195711
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1091	98.4	0.629784	7	6.88773	17	13	9.3	1089.2	6.8	0.568746

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 84

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 15.200 4.550 6.400 5.200 8.500 5.850 9.700 6.550 8.200 6.850 5.300 7.350 13.800 8.150 4.300 8.450 5.300 8.650 5.300 9.300 18.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.860 17.600 4.658 17.100 4.912 1.200 5.218 1.900 5.336 1.100 5.631 8.900 5.729 14.600 5.822 11.300 5.879 0 6.000 1.400 6.364 0.800 6.523 9.100 6.681 11.700 7.729 3.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 14 Return to Lane Navigator
3.500 16.200 4.550 6.500 5.200 7.600 5.850 9.500 6.550 7.900 6.850 5.500 7.350 13.100 8.150 3.700 8.450 5.200 8.650 4.700 9.300 20.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 5.200 3.848 9.000 4.550 8.800 4.849 0.800 5.200 0.900 5.236 2.300 5.319 0.700 5.604 2.700 5.644 0.400 5.703 7.200 5.795 6.200 5.850 1.900 5.985 0.600 6.371 0.500 6.550 4.100 6.731 4.000 6.850 2.900 7.350 3.600 7.773 1.800 8.150 4.100 8.450 5.000 8.650 24.800 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 23 Return to Lane Navigator
3.812 17.300 4.550 17.200 4.808 0.900 5.142 1.700 5.295 1.100 5.590 8.300 5.689 15.800 5.780 9.400 5.840 0 5.972 1.400 6.349 0.800 6.511 10.600 6.680 12.200 7.727 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 14 Return to Lane Navigator
3.500 15.200 4.550 6.500 5.200 8.000 5.850 9.600 6.550 8.300 6.850 5.400 7.350 16.200 8.150 3.700 8.450 5.500 8.650 4.700 9.300 17.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Great Barracuda ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Great Barracuda ---- Go to Display Gel Lane Navigator.